Clermont O, Bonacorsi S, Bingen E
Service de Microbiologie, Laboratoire d'études de génétique bactérienne dans les infections de l'enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, 48 Blvd. Sérurier, 75395 Paris Cedex 19, France.
FEMS Microbiol Lett. 2001 Mar 15;196(2):153-7. doi: 10.1111/j.1574-6968.2001.tb10557.x.
The high-pathogenicity island (HPI) present in pathogenic Yersinia and encoding the siderophore yersiniabactin, has recently been identified in the asnT tRNA region of various Escherichia coli pathotypes, especially those responsible for bacteremia and urosepsis. Most E. coli strains causing such extra-intestinal infections belong to phylogenetic groups B2 and D. In this study we investigated (i) the distribution and localization of HPI among the different E. coli phylogenetic groups, using the ECOR reference collection; and (ii) the prevalence of HPI among a set of 124 phylogenetically characterized E. coli strains responsible for neonatal meningitis. Ninety-three percent of the ECOR strains belonging to groups B2 and D harbored HPI. In contrast, the island was present in 32% and 25% of strains belonging to groups A and B1, respectively, which are considered to be non-pathogenic. HPI was found in 100% of the neonatal meningitis strains, 13 of which belonged to groups A and B1, suggesting that HPI might contain virulent factors required for the development of neonatal meningitis. Moreover, we observed for the first time that HPI can be inserted in a site different from the asnT tRNA region.
存在于致病性耶尔森氏菌中并编码铁载体耶尔森菌素的高致病性岛（HPI），最近在各种大肠杆菌致病型的asnT tRNA区域中被发现，尤其是那些导致菌血症和泌尿道感染的致病型。大多数引起此类肠外感染的大肠杆菌菌株属于系统发育群B2和D。在本研究中，我们调查了：（i）使用ECOR参考菌株集，研究HPI在不同大肠杆菌系统发育群中的分布和定位；（ii）在一组124株经系统发育特征鉴定的、导致新生儿脑膜炎的大肠杆菌菌株中HPI的流行情况。属于B2和D群的ECOR菌株中有93%携带HPI。相比之下，该岛分别存在于属于被认为无致病性的A群和B1群的32%和25%的菌株中。在100%的新生儿脑膜炎菌株中发现了HPI，其中13株属于A群和B1群，这表明HPI可能包含新生儿脑膜炎发病所需的毒力因子。此外，我们首次观察到HPI可以插入到与asnT tRNA区域不同的位点。
