藤黄微球菌菌株16S - 23S rDNA内部间隔区的结构分析与遗传变异
Structural analysis and genetic variation of the 16S-23S rDNA internal spacer region from Micrococcus luteus strains.
作者信息
Haga S, Hirano Y, Murayama O, Millar B C, Moore J E, Matsuda M
机构信息
Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
出版信息
Lett Appl Microbiol. 2003;37(4):314-7. doi: 10.1046/j.1472-765x.2003.01399.x.
AIMS
To clone and sequence the 16S-23S ribosomal DNA (rDNA) internal spacer region (ISR) from Micrococcus luteus.
METHODS AND RESULTS
The primer pair for 16S-23S rDNA ISR amplified a fragment of about 850 bp in length for two strains, JCM3347 and JCM3348 and a fragment of about 790 bp for a strain, ATCC9341. After sequencing the ISRs were identified by the comparison of the ISRs and the flanking regions of ISR.
CONCLUSIONS
Although the sequence difference of the ISR occurred at only one position between the two JCM strains, the highly variable length (440 and 370 bp) and sequence similarity (about 40%) were demonstrated between the ISRs of the two JCM strains and a ATCC strain.
SIGNIFICANCE AND IMPACT OF THE STUDY
A CCTCCT sequence was first detected at the 3'-end of the 16S rDNA of the three strains. Moreover, highly similar sequence to the 21-bp region containing a putative rRNA processing site was observed in the ISR of the three strains. Interestingly, no intercistronic tRNAs were demonstrated in the ISRs from the three strains.
目的
克隆并测序藤黄微球菌的16S - 23S核糖体DNA（rDNA）间隔区（ISR）。
方法与结果
对于两株菌JCM3347和JCM3348，16S - 23S rDNA ISR的引物对扩增出长度约为850 bp的片段，对于一株菌ATCC9341扩增出长度约为790 bp的片段。对ISR进行测序后，通过比较ISR及其侧翼区域来鉴定。
结论
尽管两株JCM菌之间的ISR序列差异仅出现在一个位置，但两株JCM菌与一株ATCC菌的ISR之间显示出高度可变的长度（440和370 bp）和序列相似性（约40%）。
研究的意义与影响
首次在三株菌的16S rDNA的3'端检测到CCTCCT序列。此外，在三株菌的ISR中观察到与包含一个假定rRNA加工位点的21 bp区域高度相似的序列。有趣的是，在三株菌的ISR中未发现顺反子间tRNA。
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