Taylor R K, Hale C N
The Horticulture and Food Research Institute of New Zealand Ltd, Auckland, New Zealand.
Lett Appl Microbiol. 2003;37(4):340-3. doi: 10.1046/j.1472-765x.2003.01407.x.
To determine the effect of cold storage on the survival of Erwinia amylovora.
The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low temperatures. In contrast, populations of E. amylovora on tissue in the apple calyx decreased during cold storage.
Erwinia amylovora has the ability, in nutrient media, to multiply at low temperatures. However, populations of E. amylovora on tissue in the apple calyx decrease with the time spent in cold storage.
Cold storage of apples will provide assurance that mature fruit from orchards, free of fire blight, or even with low levels of fire blight, may be exported with a negligible risk of introducing the disease into countries free of fire blight.
确定冷藏对梨火疫病菌存活的影响。
在2℃储存期间评估梨火疫病菌的存活情况。接种到磷酸盐缓冲盐水中的梨火疫病菌数量保持稳定，而在营养培养基中，其数量在低温下增加。相比之下，苹果花萼组织上的梨火疫病菌数量在冷藏期间减少。
梨火疫病菌在营养培养基中有在低温下繁殖的能力。然而，苹果花萼组织上的梨火疫病菌数量会随着冷藏时间的延长而减少。
苹果冷藏将确保果园中无火疫病或甚至火疫病水平较低的成熟果实出口时，将该病害引入无火疫病国家的风险可忽略不计。
