Wu Xiao-Ming, Zhou Yi-Kai, Ren Shu, Hao Qiao-Ling
Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, PR China.
Ai Zheng. 2003 Sep;22(9):912-5.
BACKGROUND & OBJECTIVE: A-463 bp G/A polymorphism is located in the promoter region of myeloperoxidase (MPO) gene was found to be associated with lung cancer susceptibility; however, this association in Chinese population remained unclear. The aim of this study was designed to explore this association in Chinese population.
MPO genotypes in 98 cases of primary lung cancer and 112 persons of healthy control were detected using PCR-restriction fragment length polymorphism assay(PCR-RFLP) in a case-control molecular epidemiology study. The association between this gene polymorphism and the risk of lung cancer in Chinese population was examined through comparing odds ratio(OR) and 95% confidence interval(CI) between two groups.
In healthy control group, the frequencies of persons carrying G/G, G/A, and A/A genotypes were 47.3%, 42.9%, and 9.8%, respectively. In cancer group, the frequencies of the persons carrying above three genotypes were 63.3%, 33.7%, and 3.0%, respectively. There was no significant difference of the frequencies of heterozygote G/A between two groups (P >0.05). However, The A/A genotype was found significantly higher in cancer group than that in healthy control group(P< 0.025). The risk of lung cancer for person carrying at least one A allele was only 52% of the persons carrying G/G genotype (95% CI 0.29-0.93). In smoking population, allele A showed to be a significant protective factor (OR=0.41,P< 0.025), while such protective role was not observed in non-smoking group(P >0.25).
MPO gene polymorphism was associated with susceptibility of lung cancer in Chinese population. The risk of lung cancer was decreased in the persons carrying allele A.
髓过氧化物酶（MPO）基因启动子区域存在一个463bp的G/A多态性，已发现其与肺癌易感性相关；然而，该多态性在中国人群中的相关性尚不清楚。本研究旨在探讨其在中国人群中的相关性。
采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法，对98例原发性肺癌患者和112名健康对照者进行MPO基因分型，进行病例对照分子流行病学研究。通过比较两组的比值比（OR）和95%置信区间（CI），研究该基因多态性与中国人群肺癌风险的相关性。
在健康对照组中，携带G/G、G/A和A/A基因型的频率分别为47.3%、42.9%和9.8%。在癌症组中，携带上述三种基因型的频率分别为63.3%、33.7%和3.0%。两组杂合子G/A的频率无显著差异（P>0.05）。然而，发现癌症组中A/A基因型显著高于健康对照组（P<0.025）。携带至少一个A等位基因的人患肺癌的风险仅为携带G/G基因型者的52%（95%CI 0.29-0.93）。在吸烟人群中，等位基因A显示为显著的保护因素（OR=0.41，P<0.025），而在非吸烟组中未观察到这种保护作用（P>0.25）。
MPO基因多态性与中国人群肺癌易感性相关。携带等位基因A的人患肺癌的风险降低。
