Grove S, Høie S, Evensen Ø
Department of Fish Health, National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway.
Fish Shellfish Immunol. 2003 Oct;15(4):349-58. doi: 10.1016/s1050-4648(02)00184-5.
In this study we report the differences in distribution and retention of Aeromonas salmonicida antigens after vaccination with two different vaccines. Parr of Atlantic salmon (Salmo salar) were given intraperitoneal injections of either a commercial, monovalent furunculosis vaccine (Apoject) or live, attenuated A. salmonicida (DeltaaroA). Fish were sampled at weeks 2, 4 and 12 post-vaccination and head kidney and spleen were collected. Presence of LPS and 16S rDNA in isolated leukocytes were investigated by immunocytochemistry and polymerase chain reaction (PCR).16S rDNA was detected in head kidney and spleen of all DeltaaroA vaccinated and most Apoject-vaccinated fish at weeks 2 and 4. At week 12, 16S rDNA was detected in none of the DeltaaroA vaccinated fish, but it was detected in head kidney of 75% of Apoject-vaccinated fish. LPS was detected in both vaccination groups at all sampling times, but most frequently in the DeltaaroA vaccinated fish (in head kidney 75-83% vs. 50%, in spleen 58-67% vs. 17-25%).
在本研究中，我们报告了用两种不同疫苗接种后，杀鲑气单胞菌抗原在分布和留存方面的差异。给大西洋鲑幼鱼（Salmo salar）腹腔注射市售单价疖疮病疫苗（Apoject）或减毒活杀鲑气单胞菌（DeltaaroA）。在接种疫苗后的第2周、第4周和第12周对鱼进行采样，并采集头肾和脾脏。通过免疫细胞化学和聚合酶链反应（PCR）研究分离白细胞中脂多糖（LPS）和16S核糖体DNA（rDNA）的存在情况。在第2周和第4周，在所有接种DeltaaroA疫苗的鱼以及大多数接种Apoject疫苗的鱼的头肾和脾脏中检测到16S rDNA。在第12周，接种DeltaaroA疫苗的鱼中均未检测到16S rDNA，但在75%接种Apoject疫苗的鱼的头肾中检测到。在所有采样时间，两个接种组均检测到LPS，但在接种DeltaaroA疫苗的鱼中最为常见（头肾中为75 - 83%对50%，脾脏中为58 - 67%对17 - 25%）。
