Mateo Gema, Corral Mercedes, Almeida Julia, López-Berges Consuelo, Nieto maría Jesús, García-Marcos Antonia, Vázquez Lourdes, del Cañizo Consuelo, Orfao Alberto, San Miguel Jesús F
Hematology Department, University of Salamanca, Spain.
Haematologica. 2003 Sep;88(9):1013-21.
Four-color multiparameter immunophenotyping has recently proven to be an attractive technique for evaluating the plasma cell (PC) compartment since it allows discrimination between myelomatous and normal PC. This study was designed to investigate: i) whether peripheral blood is less contaminated than bone marrow as a source for an autologous transplant; ii) the effect of growth factors on mobilizing myelomatous PC into peripheral blood; iii) the degree of contamination by myelomatous PC in apheresis samples; and iv) whether the number of PC increases during the last days of apheresis.
Using 4-color antigen staining we investigated the composition of the PC compartment in 90 apheresis products from 40 patients with MM; in 17 cases bone marrow and peripheral blood samples were also simultaneously evaluated.
(i) All pre-mobilization bone marrow samples analyzed were always contaminated with myelomatous PC whereas only 41% of the post-mobilization peripheral blood samples were contaminated. Moreover, the use of peripheral blood would lead to a reduction of >5x10(5) infused myelomatous PC; (ii) mobilization with cytokines increased the number of circulating PC, generally because of an expansion of the normal PC population; (iii) forty-eight percent of all peripheral blood stem cell harvests were contaminated with myelomatous PC, although normal PC usually represented the predominant population; (iv) no significant changes were observed in the amount of contaminating myelomatous PC during the first three days of apheresis.
Multiparameter immunophenotyping is a useful approach for investigating the PC compartment in apheresis products.
四色多参数免疫表型分析最近已被证明是一种评估浆细胞（PC）区室的有吸引力的技术，因为它能够区分骨髓瘤性PC和正常PC。本研究旨在调查：i）作为自体移植的来源，外周血是否比骨髓污染更少；ii）生长因子对将骨髓瘤性PC动员到外周血中的作用；iii）单采样本中骨髓瘤性PC的污染程度；以及iv）单采最后几天PC数量是否增加。
我们使用四色抗原染色研究了40例多发性骨髓瘤（MM）患者的90份单采产品中PC区室的组成；17例同时评估了骨髓和外周血样本。
（i）所有分析的动员前骨髓样本均始终被骨髓瘤性PC污染，而动员后外周血样本只有41%被污染。此外，使用外周血将导致注入的骨髓瘤性PC减少>5×10⁵；（ii）细胞因子动员增加了循环PC的数量，通常是由于正常PC群体的扩增；（iii）所有外周血干细胞采集物中有48%被骨髓瘤性PC污染，尽管正常PC通常占主要群体；（iv）在单采的前三天，污染的骨髓瘤性PC数量未观察到显著变化。
多参数免疫表型分析是研究单采产品中PC区室的一种有用方法。
