乙型肝炎患者外周血单个核细胞表面T淋巴细胞亚群及可溶性白细胞介素-2受体的检测
Detection of T lymphocyte subsets and mIL-2R on surface of PBMC in patients with hepatitis B.
作者信息
Wang Ke-Xia, Peng Jiang-Long, Wang Xue-Feng, Tian Ye, Wang Jian, Li Chao-Pin
机构信息
School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China.
出版信息
World J Gastroenterol. 2003 Sep;9(9):2017-20. doi: 10.3748/wjg.v9.i9.2017.
AIM
To study the levels of T lymphocyte subsets and membrane interleukin-2 receptor (mIL-2R) on surface of peripheral blood mononuclear cells (PBMCs) of patients with hepatitis B and its role in the pathogenesis of hepatitis B.
METHODS
The levels of T lymphocyte subsets and mIL-2R in PBMC before and after being stimulated with PHA were detected by biotin-streptavidin (BSA) technique in 196 cases of hepatitis B.
RESULTS
In patients with hepatitis B, the levels of CD(3)(+), CD(4)(+) cells, and the ratio of CD(4)(+) cells/CD(8)(+) cells were lower, but the level of CD(8)(+) cells was higher than those in normal controls (42.20+/-6.01 vs 65.96+/-6.54, 38.17+/-5.93 vs 41.73+/-6.40, 0.91+/-0.28 vs 1.44+/-0.31, 39.86+/-6.36 vs 30.02+/-4.54, P<0.01). The total expression level of mIL-2R in PBMC before and after being stimulated with PHA was also lower than those in normal controls (3.47+/-1.55 vs 4.52+/-1.49, 34.03+/-2.94 vs 37.95+/-3.00, P<0.01). In all the patients with hepatitis B, the levels of T lymphocyte subsets and mIL-2R in PBMC with HBV-DNA (+) were lower than those with HBV-DNA (-), which were significantly different (39.57+/-7.11 vs 44.36+/-5.43, 34.36+/-7.16 vs 40.75+/-5.87, 37.82+/-6.54 vs 41.72+/-6.21, 0.88+/-0.33 vs 0.99+/-0.27, 2.82+/-1.62 vs 3.85+/-1.47, 31.56+/-3.00 vs 35.84+/-2.83, P<0.01). In addition, the levels of CD(3)(+), CD(4)(+), CD(8)(+) cells, the ratio of CD(4)(+) cells /CD(8)(+) cells and mIL-2R among different courses of hepatitis B were all significantly different (F=3 723.18, P<0.01. F=130.43, P<0.01. F=54.01, P<0.01. F=2.99, P<0.05. F=7.16, P<0.01).
CONCLUSION
Both cellular and humoral immune functions are obviously in disorder in patients with hepatitis B, which might be closely associated with the chronicity in patients.
目的
研究乙型肝炎患者外周血单个核细胞（PBMC）表面T淋巴细胞亚群及膜白细胞介素-2受体（mIL-2R）水平及其在乙型肝炎发病机制中的作用。
方法
采用生物素-链霉亲和素（BSA）技术检测196例乙型肝炎患者PHA刺激前后PBMC中T淋巴细胞亚群及mIL-2R水平。
结果
乙型肝炎患者CD(3)(+)、CD(4)(+)细胞水平及CD(4)(+)细胞/CD(8)(+)细胞比值低于正常对照组，而CD(8)(+)细胞水平高于正常对照组（42.20±6.01对65.96±6.54，38.17±5.93对41.73±6.40，0.91±0.28对1.44±0.31，39.86±6.36对30.02±4.54，P<0.01）。PHA刺激前后PBMC中mIL-2R总表达水平也低于正常对照组（3.47±1.55对4.52±1.49，34.03±2.94对37.95±3.00，P<0.01）。所有乙型肝炎患者中，HBV-DNA(+)的PBMC中T淋巴细胞亚群及mIL-2R水平低于HBV-DNA(-)者，差异有统计学意义（39.57±7.11对44.36±5.43，34.36±7.16对40.75±5.87，37.82±6.54对41.72±6.21，0.88±0.33对0.99±0.27，2.82±1.62对3.85±1.47，31.56±3.00对35.84±2.83，P<0.01）。此外，不同病程乙型肝炎患者CD(3)(+)、CD(4)(+)、CD(8)(+)细胞水平、CD(4)(+)细胞/CD(8)(+)细胞比值及mIL-2R均有显著差异（F=3723.18，P<0.01；F=13,43，P<0.01；F=54.01，P<0.01；F=2.99，P<0.05；F=7.16，P<0.01）。
结论
乙型肝炎患者细胞免疫和体液免疫功能均明显紊乱，这可能与患者的慢性化密切相关。
Zotero 插件