[猪苓发酵物对大鼠中性粒细胞体外释放白三烯B4、C4和D4的抑制作用]

[Inhibition of Cryptoporus volvatus ferment substance on release of leukotriene B4, C4 and D4 from neutrophils in rats in vitro].

作者信息

Jin Sai-hong, Xie Qiang-min, Chen Ji-qiang

机构信息

Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, College of Medicine, Zhejiang University, Hangzhou 310031, China.

出版信息

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Aug;32(4):292-5. doi: 10.3785/j.issn.1008-9292.2003.04.006.

DOI:10.3785/j.issn.1008-9292.2003.04.006

PMID:12970928

Abstract

OBJECTIVE

To study inhibitory the effects of Cryptoporus volvatus ferment substance(CVFS) on leukotriene production in vitro from neutrophils in rats.

METHODS

Neutrophil aggregation was induced by intraperitoneal injection of glycogen in rats. After 16 h, intraperitoneal lavage fluid(PLF) was collected and neutrophils were removed. Neutrophils were stimulated by calcium ionophore A23187 in vitro to produce leukotriene B(4), C(4), D(4). The concentrations of leukotriene B(4), C(4) and D(4) were measured by reversed-phase high-performance liquid chromatography(HPLC).

RESULT

CVFS at 0.25, 1, 4 mg x L(-1)decreased leukotriene B(4), C(4), D(4) release from neutrophils in a concentration-dependent manner. Inhibitory rate of CVFS 0.25, 1, 4 mg x L(-1 )on A23187-induced leukotriene B(4) production was 27.4%, 54.2% and 78.8%(P<0.05), respectively. Inhibitory rate of leukotriene C(4) production was 65.1%, 74.3 and 79.0%(P<0.05), respectively. Inhibitory rate of leukotriene D(4) production was 55.6%, 60.9% and 72.8%(P<0.05), respectively.

CONCLUSION

The results suggest that suppression of leukotriene release may be a mechanism of the anti-inflammation and anti-asthma effects of CVFS.

摘要

目的

研究茯苓发酵产物（CVFS）对大鼠中性粒细胞体外白三烯生成的抑制作用。

方法

通过腹腔注射糖原诱导大鼠中性粒细胞聚集。16小时后，收集腹腔灌洗液（PLF）并去除中性粒细胞。体外使用钙离子载体A23187刺激中性粒细胞产生白三烯B4、C4、D4。采用反相高效液相色谱法（HPLC）测定白三烯B4、C4和D4的浓度。

结果

CVFS在0.25、1、4mg·L-1时可呈浓度依赖性降低中性粒细胞白三烯B4、C4、D4的释放。CVFS 0.25、1、4mg·L-1对A23187诱导的白三烯B4生成的抑制率分别为27.4%、54.2%和78.8%（P<0.05）。对白三烯C4生成的抑制率分别为65.1%、74.3%和79.0%（P<0.05）。对白三烯D4生成的抑制率分别为55.6%、60.9%和72.8%（P<0.05）。