Athanasas-Platsis Stavrosia, Zhang Bing, Hillyard Narelle C, Cavanagh Alice C, Csurhes Peter A, Morton Halle, McCombe Pamela A
Department of Medicine, The University of Queensland, Royal Brisbane Hospital, Queensland 4029, Australia.
J Neurol Sci. 2003 Oct 15;214(1-2):27-36. doi: 10.1016/s0022-510x(03)00170-9.
Early pregnancy factor (EPF) is a secreted protein with immunosuppressive and growth factor properties that has been shown to suppress acute experimental autoimmune encephalomyelitis (EAE) induced with myelin basic protein (MBP) in Lewis rats. EAE is associated with infiltration of the central nervous system (CNS) with inflammatory cells. Spontaneous recovery involves the loss of T lymphocytes from the CNS and the selective apoptosis of Vbeta8.2+ cells. In the present study, T cell, macrophage (CD11b/c+) and B cell (CD45RA+) populations in spinal cord and popliteal lymph nodes (LN) of Lewis rats with EAE were quantitated and apoptosis was studied. Rats were treated with EPF or vehicle. Following treatment on day 14 after inoculation with MBP, neither 1 x 100 microg nor 2 x 100 microg doses of EPF affected the total number of cells infiltrating the spinal cord on day 15, although the higher dose caused a decrease in the number of CD5+ and CD11b/c+ cells. Treatment with 2 x 100 microg/day from days 10 to 14 decreased the total number of infiltrating cells, and the numbers of CD5+, CD11b/c+ and CD45RA+ cells. Apoptosis was unaffected. No alteration on the number or type of inflammatory cells in the popliteal LN was observed after treatment on days 10-14. However, treatment with EPF from days 0 to 11 increased the total number of T and B cells and CD5+ T cells found on day 12 in the LN. Similarly, there was an increase in the frequency of MBP-reactive cells in the LN as determined by limiting dilution analysis. These results suggest that EPF treatment reduces the numbers of lymphocytes and macrophages in the CNS, possibly through an effect on cell trafficking.
早孕因子（EPF）是一种具有免疫抑制和生长因子特性的分泌蛋白，已被证明可抑制Lewis大鼠中由髓鞘碱性蛋白（MBP）诱导的急性实验性自身免疫性脑脊髓炎（EAE）。EAE与中枢神经系统（CNS）中炎性细胞的浸润有关。自发恢复涉及中枢神经系统中T淋巴细胞的丢失以及Vbeta8.2 +细胞的选择性凋亡。在本研究中，对患有EAE的Lewis大鼠脊髓和腘窝淋巴结（LN）中的T细胞、巨噬细胞（CD11b / c +）和B细胞（CD45RA +）群体进行了定量，并研究了细胞凋亡情况。大鼠接受EPF或赋形剂治疗。在接种MBP后第14天进行治疗，尽管较高剂量导致CD5 +和CD11b / c +细胞数量减少，但1×100μg和2×100μg剂量的EPF均未影响第15天脊髓中浸润细胞的总数。从第10天到第14天每天用2×100μg治疗可减少浸润细胞的总数以及CD5 +、CD11b / c +和CD45RA +细胞的数量。细胞凋亡未受影响。在第10 - 14天治疗后，未观察到腘窝淋巴结中炎性细胞的数量或类型有改变。然而，从第0天到第11天用EPF治疗可增加第12天在淋巴结中发现的T细胞、B细胞和CD5 + T细胞的总数。同样，通过有限稀释分析确定，淋巴结中MBP反应性细胞的频率有所增加。这些结果表明，EPF治疗可能通过影响细胞运输来减少中枢神经系统中淋巴细胞和巨噬细胞的数量。
