García Pérez B, Ayala I, Castells M T, Madrid J F, Ortega M R, Ortega J V, Ballesta J, Fernández Pardo J, Valdés M
Clinical Universitary Hospital, Virgen de la Arrixaca, Murcia, Spain.
Histol Histopathol. 2003 Oct;18(4):1027-33. doi: 10.14670/HH-18.1027.
Calcium appears to be involved in many of the cellular events which are thought to be important in atherogenesis. Calcium channel blockers have been shown to reduce arterial lipid accumulation in animals without altering serum cholesterol. Avian models of atherosclerosis offer economic and technical advantages over mammalian models. In this study, we examine the effects of nifedipine, verapamil and diltiazem at clinical and higher doses, on the extent of atherosclerosis of egg-fed chickens. In order to assess the extent of atherosclerosis quantitatively, the aortic lesions of the thoracic and abdominal aorta, aortic arch and supraaortic regions were measured by planimetry. Atherosclerotic lesions were evaluated histologically. Statistically significant reductions in the lipid deposition of the aorta were found in all the treated groups. The extent and distribution of atherosclerotic lesions were decreased in a significant way by verapamil, nifedipine and diltiazem. The higher the dosage used, the higher the regression of the atherosclerotic lesions. At clinical dosage, nifedipine showed the highest decrease of the lesions. In addition, the chicken atherosclerosis model has proved itself useful and very suitable for in vivo drug intervention studies.
钙似乎参与了许多被认为在动脉粥样硬化形成过程中很重要的细胞活动。钙通道阻滞剂已被证明能减少动物动脉脂质蓄积，而不改变血清胆固醇水平。与哺乳动物模型相比，鸟类动脉粥样硬化模型具有经济和技术上的优势。在本研究中，我们研究了临床剂量及更高剂量的硝苯地平、维拉帕米和地尔硫䓬对喂食鸡蛋的鸡的动脉粥样硬化程度的影响。为了定量评估动脉粥样硬化的程度，通过平面测量法测量胸主动脉、腹主动脉、主动脉弓和主动脉上区域的主动脉病变。对动脉粥样硬化病变进行组织学评估。在所有治疗组中均发现主动脉脂质沉积有统计学意义的减少。维拉帕米、硝苯地平和地尔硫䓬显著降低了动脉粥样硬化病变的程度和分布。使用的剂量越高，动脉粥样硬化病变的消退程度越高。在临床剂量下，硝苯地平对病变的降低幅度最大。此外，鸡动脉粥样硬化模型已证明其自身有用且非常适合体内药物干预研究。
