Kondomerkos D J, Kalamidas S A, Kotoulas O B
Department of Anatomy, Histology and Embryology, Medical School, University of Ioannina, Ioannina, Greece.
Histol Histopathol. 2003 Oct;18(4):1103-13. doi: 10.14670/HH-18.1103.
Peritoneal and bronchoalveolar macrophages activated in vitro by endotoxin, exhibit alterations in the acid phosphatase activity of cell lysates when certain hormones or autacoids are present in the culture medium. They also show morphological changes concerning general appearance and acid phosphatase cytochemistry. Certain agents known to increase the intracellular levels of cyclic AMP, such as dopamine and prostaglandin E2, decreased this enzyme activity in the lysates of peritoneal macrophages. Adrenalin had no effect on this activity at 14 hours, but was found to increase the activity in the culture medium at the initial hours of incubation. Glucagon decreased whereas insulin increased acid phosphatase activity in bronchoalveolar macrophages. Serotonin or histamine, known to activate phospholipase C, increased this activity in peritoneal or bronchoalveolar macrophages. The results of this study, taken together with previously published data (Kondomerkos et al., 2003), suggest that hormones and autacoids may control certain parameters of macrophage activation including acid phosphatase activity.
当培养基中存在某些激素或自分泌物质时，体外被内毒素激活的腹腔巨噬细胞和支气管肺泡巨噬细胞，其细胞裂解物中的酸性磷酸酶活性会发生改变。它们在整体外观和酸性磷酸酶细胞化学方面也呈现出形态变化。某些已知能提高细胞内环磷酸腺苷水平的物质，如多巴胺和前列腺素E2，会降低腹腔巨噬细胞裂解物中的这种酶活性。肾上腺素在14小时时对该活性无影响，但在孵育初期被发现会增加培养基中的活性。胰高血糖素会降低支气管肺泡巨噬细胞中的酸性磷酸酶活性，而胰岛素则会增加其活性。已知能激活磷脂酶C的血清素或组胺，会增加腹腔或支气管肺泡巨噬细胞中的这种活性。这项研究的结果与先前发表的数据（Kondomerkos等人，2003年）相结合，表明激素和自分泌物质可能控制巨噬细胞激活的某些参数，包括酸性磷酸酶活性。
