Catarcini Paolo, Fanali Salvatore, Presutti Carla, D'Acquarica Ilaria, Gasparrini Francesco
Istituto di Metodologie Chimiche, C.N.R., Monterotondo Scalo (Roma), Italy.
Electrophoresis. 2003 Sep;24(17):3000-5. doi: 10.1002/elps.200305504.
Different types of fused-silica capillaries of 75 microm inside diameter (ID) were packed, namely type A and B, and evaluated for the direct resolution of racemates of several basic compounds by enantioselective capillary electrochromatography (e-CEC). Type A was packed with a chiral stationary phase (CSP) containing teicoplanin (TE) mixed with silica microparticles (3:1 w/w) while type B contained only the TE-CSP. In both cases, particles of different sizes (3.5 and 5 microm ID) were employed. A polar-organic mobile phase containing methanol-acetonitrile (60-40% v/v and 0.05% w/v ammonium acetate was used. Several beta-blockers (alprenolol, oxprenolol, metoprolol, pindolol, salbutamol, propranolol, atenolol, acebutolol) were baseline-enantioresolved with both capillary types, in very short times.
填充了内径为75微米的不同类型熔融石英毛细管，即A型和B型，并通过对映体选择性毛细管电色谱法（e-CEC）评估了几种碱性化合物外消旋体的直接拆分效果。A型填充有含有替考拉宁（TE）与二氧化硅微粒（3:1 w/w）混合的手性固定相（CSP），而B型仅含有TE-CSP。在这两种情况下，均使用了不同尺寸（内径3.5和5微米）的颗粒。使用了含有甲醇-乙腈（60-40% v/v）和0.05% w/v醋酸铵的极性有机流动相。几种β受体阻滞剂（阿普洛尔、氧烯洛尔、美托洛尔、吲哚洛尔、沙丁胺醇、普萘洛尔、阿替洛尔、醋丁洛尔）在两种毛细管类型中均在非常短的时间内实现了基线对映体拆分。
