Guo Yanping, Zhou Huifen, Zeng Guanghui, Zhang Liangcheng
Department of Horticulture, Zhejiang University, Hangzhou 310029, China.
Ying Yong Sheng Tai Xue Bao. 2003 Jun;14(6):867-70.
Table ADC-225-MK3 CO2 gas analyzer and PAM-2000 portable fluorescence measurement system were used to measure the net photosynthetic rate (Pn), initial fluorescence (Fo), maximal photochemical efficiency of PS II (Fv/Fm) and electron transport rate (ETR) of satsuma mandarin (Citrus unshiu Marc.) and navel orange (C. sinensis Osbeck) leaves. The results showed that exposure of citrus plants to high temperature (38-40 degrees C) led to a lowering of Pn, Fv/Fm, and ETR, whereas Fo increased. After exposure to high temperature for 25 days, compared with normal temperature (25 degrees C), the Pn of satsuma mandarin and navel orange leaves decreased by 55.6% and 39.8%, Fv/Fm decreased by 22.0% and 6.7% and ETR reduced by 55.0% and 41.5%, respectively. On the other hand, Fo increased 113.8% and 14.9%, respectively. With subsequent transfer to the 25 degrees C culture room for 10 days, Pn, Fv/Fm, Fo and ETR recovered significantly. These results demonstrated that the reduction of Pn in Citrus was related to the inactivation of PSII reaction center.
使用Table ADC - 225 - MK3型二氧化碳气体分析仪和PAM - 2000便携式荧光测量系统，测定温州蜜柑（Citrus unshiu Marc.）和脐橙（C. sinensis Osbeck）叶片的净光合速率（Pn）、初始荧光（Fo）、PSⅡ的最大光化学效率（Fv/Fm）和电子传递速率（ETR）。结果表明，将柑橘植株置于高温（38 - 40摄氏度）环境中会导致Pn、Fv/Fm和ETR降低，而Fo升高。在高温处理25天后，与常温（25摄氏度）相比，温州蜜柑和脐橙叶片的Pn分别下降了55.6%和39.8%，Fv/Fm分别下降了22.0%和6.7%，ETR分别降低了55.0%和41.5%。另一方面，Fo分别升高了113.8%和14.9%。随后将植株转移到25摄氏度的培养室中培养10天，Pn、Fv/Fm、Fo和ETR显著恢复。这些结果表明，柑橘中Pn的降低与PSII反应中心的失活有关。
