丁酸钠诱导HeLa细胞选择性凋亡及其机制

[Selective apoptosis in HeLa cells induced by sodium butyrate and its mechanism].

作者信息

Xi Ling, Wu Ming-fu, Feng Yong-dong, Wang Wei, Lu Yun-ping, Zhou Jian-feng, Ma Ding

机构信息

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Aug;25(4):401-5.

PMID:12974082

Abstract

OBJECTIVE

To investigate apoptosis induced by sodium butyrate in cervix cancer cell line HeLa and primary human embryo lung fibroblasts and its mechanism.

METHODS

Cell apoptosis was assessed by morphology, cell viability, DNA fragmentation, the percentage of sub-G1 cells and phosphatidylserine (PS) externalization. The effects of sodium butyrate on transcription of Bax and Bcl-2 was analyzed by RT-PCR.

RESULTS

Sodium butyrate inhibited proliferation in a time and dose-dependant manner. The inhibition of proliferation in HeLa cells was more significant than that in primary human embryo lung fibroblasts. DNA fragmentation, sub-G1 peak and AnnexinV/PI by flow cytometry showed very high apoptosis rates in HeLa cells 72 hours after treated with sodium butyrate, while pretty low in primary human embryo lung fibroblasts. RT-PCR showed sodium butyrate had little effects on transcription of Bax and Bcl-2 in HeLa cells.

CONCLUSION

Sodium butyrate can induce apoptosis in HeLa cells without changing the expression of Bax and Bcl-2. Sodium butyrate comparatively has little effects on fibroblasts.

摘要

目的

研究丁酸钠对宫颈癌HeLa细胞系及原代人胚肺成纤维细胞凋亡的诱导作用及其机制。

方法

通过形态学、细胞活力、DNA片段化、亚G1期细胞百分比及磷脂酰丝氨酸（PS）外翻评估细胞凋亡。采用逆转录聚合酶链反应（RT-PCR）分析丁酸钠对Bax和Bcl-2转录的影响。

结果

丁酸钠以时间和剂量依赖性方式抑制细胞增殖。丁酸钠对HeLa细胞增殖的抑制作用比对原代人胚肺成纤维细胞更显著。DNA片段化、亚G1峰及流式细胞术检测的膜联蛋白V/碘化丙啶（AnnexinV/PI）结果显示，丁酸钠处理72小时后HeLa细胞凋亡率很高，而原代人胚肺成纤维细胞凋亡率很低。RT-PCR结果显示丁酸钠对HeLa细胞中Bax和Bcl-2的转录影响很小。