Park K S, Choi S Y, Park M H, Tokunaga K
Department of Biology, Sung Shin Women's University, Seoul, Korea.
Jpn J Hum Genet. 1992 Dec;37(4):285-92. doi: 10.1007/BF01883319.
The analysis of genetic polymorphism in C4 was performed on EDTA-plasma from 169 healthy unrelated Koreans. Plasma samples were subjected to high-voltage agarose gel electrophoresis followed by immunofixation. C4B allotypes were further detected by a hemolytic overlay method. The allele frequencies of C4A and C4B were as follows; for C4A, C4A3 = 0.6099, C4A4 = 0.1702, C4AQ0 = 0.1525, C4A2 = 0.0461, and C4AR = 0.0213; for C4B, C4B1 = 0.6406, C4B2 = 0.2740, C4B5 = 0.0569, C4BQ0 = 0.0178, and C4BR = 0.0107. C4A3 and C4B1 were among the most common alleles at each locus. C4A6 was not detected in this study, but this allele is relatively common in both Caucasoid and Negroid populations. C4B5 is a common allele in Asian, which is rare in Caucasoids and Negroids. C4B*5 appeared to be a characteristic allele of Oriental. In the C4A locus, five individuals with duplicated allotypes (three C4A 3,3 + 2, one C4A 4,3 + 2, and one C4A 3,3 + 3) were observed, and in the C4B locus, one individual with duplicated allotype (C4B 1,1 + 1) was detected.
对169名健康、无亲缘关系的韩国人的乙二胺四乙酸（EDTA）血浆进行了C4基因多态性分析。血浆样本先进行高压琼脂糖凝胶电泳，然后进行免疫固定。通过溶血覆盖法进一步检测C4B同种异型。C4A和C4B的等位基因频率如下：对于C4A，C4A3 = 0.6099，C4A4 = 0.1702，C4AQ0 = 0.1525，C4A2 = 0.0461，C4AR = 0.0213；对于C4B，C4B1 = 0.6406，C4B2 = 0.2740，C4B5 = 0.0569，C4BQ0 = 0.0178，C4BR = 0.0107。C4A3和C4B1是每个位点最常见的等位基因之一。本研究未检测到C4A6，但该等位基因在白种人和黑种人群体中相对常见。C4B5是亚洲人群中的常见等位基因，在白种人和黑种人中罕见。C4B*5似乎是东方人的特征性等位基因。在C4A位点，观察到5名具有重复同种异型的个体（3名C4A 3,3 + 2，1名C4A 4,3 + 2，1名C4A 3,3 + 3），在C4B位点，检测到1名具有重复同种异型的个体（C4B 1,1 + 1）。
