通过23S核糖体RNA和rdxA基因分析确定日本广岛幽门螺杆菌对克拉霉素和甲硝唑的耐药率
Prevalence of Helicobacter pylori resistance to clarithromycin and metronidazole determined by 23S ribosomal RNA and rdxA gene analyses in Hiroshima, Japan.
作者信息
Hiyama Toru, Tanaka Shinji, Masuda Hiroshi, Shima Hideyuki, Kose Kazuhiro, Tuncel Handan, Ito Masanori, Kitadai Yasuhiko, Sumii Masaharu, Uemura Naomi, Yoshihara Masaharu, Shimamoto Fumio, Haruma Ken, Chayama Kazuaki
机构信息
Health Service Center, Hiroshima University, Higashihiroshima, Japan.
出版信息
J Gastroenterol Hepatol. 2003 Oct;18(10):1202-7. doi: 10.1046/j.1440-1746.2003.03140.x.
BACKGROUND AND AIMS
Resistance to antibiotics in Helicobacter pylori is increasing and becoming a serious problem in eradication treatment of H. pylori. The prevalence of H. pylori infections that are resistant to clarithromycin, metronidazole, or both were determined in H. pylori isolates in Hiroshima, Japan.
METHODS
Sixty Japanese patients with H. pylori infection were collected between 1999 and 2000. To detect the resistance to clarithromycin and metronidazole, mutations of the 23S ribosomal RNA (rRNA) and rdxA genes that are responsible for resistance in H. pylori, were examined by direct sequencing analysis.
RESULTS
Resistance to clarithromycin and metronidazole was detected in 12 (20.0%) and nine (15.0%) of the patients, respectively. Dual resistance to clarithromycin and metronidazole was detected in five (8.3%) patients.
CONCLUSION
These results indicate that the relatively high prevalence of the dual resistance in H. pylori isolates may need special attention and new therapeutic approaches in Japan.
背景与目的
幽门螺杆菌对抗生素的耐药性正在增加，成为幽门螺杆菌根除治疗中的一个严重问题。我们测定了日本广岛幽门螺杆菌分离株中对克拉霉素、甲硝唑或两者均耐药的幽门螺杆菌感染的流行情况。
方法
1999年至2000年间收集了60例日本幽门螺杆菌感染患者。为检测对克拉霉素和甲硝唑的耐药性，通过直接测序分析检测了幽门螺杆菌中负责耐药性的23S核糖体RNA（rRNA）和rdxA基因的突变。
结果
分别在12例（20.0%）和9例（15.0%）患者中检测到对克拉霉素和甲硝唑的耐药性。在5例（8.3%）患者中检测到对克拉霉素和甲硝唑的双重耐药性。
结论
这些结果表明，在日本，幽门螺杆菌分离株中双重耐药性的相对高流行率可能需要特别关注和新的治疗方法。
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