[生活方式、亚甲基四氢叶酸还原酶基因与胸苷酸合成酶基因多态性之间的相互作用与胃癌风险]

[Interactions between lifestyle, methylanetetrahydrofolate reductase gene and polymorphisms in thymidylate synthase gene with risk of stomach cancer].

作者信息

Gao Chang-ming, Wu Jian-zhong, Liu Yan-ting, Ding Jian-hua, Li Su-ping, Su Ping, Hu Xu, Kai Hai-tao, Toshiro Takezaki, Kazuo Tajima

机构信息

Department of Epidemiology, Jiangsu Province Institute of Cancer Research, Nanjing 210009, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jul;24(7):599-603.

PMID:12975018

Abstract

OBJECTIVE

To evaluate interactions between lifestyle, methylanetetrahydrofolate reductase gene (MTHFR) and polymorphisms in the 3'-untranslated region (3'-UTR) of the thymidylate synthase gene (TS) with reference to development of stomach cancer (SC).

METHODS

We conducted a case-control study with 107 cases of SC and 200 population-based controls in Huaian city of Jiangsu province, China. TS genotypes were identified by polymerase chain reaction.

RESULTS

(1) The frequencies of TS genotypes (+6 bp/+6 bp, +6 bp/-6 bp and -6 bp/-6 bp) among the cases were 5.6%, 47.7% and 46.7% and among the controls were 9.0%, 54.0% and 37.0%, respectively. Individuals identified as -6 bp/-6 bp genotype had a slightly higher risk for SC than those individuals with +6 bp alleles (the crude OR = 1.49, 95% CI: 0.90 - 2.47; adjusted OR = 1.36, 95% CI: 1.00 - 1.78, P = 0.047). (2) Individuals having TS -6 bp/-6 bp genotype and having smoking habit were at a significantly higher risk of developing SC (adjusted OR = 2.79, 95% CI: 1.51 - 5.18) compared with those who had +6 bp alleles with no smoking habit. Individuals having TS -6 bp/-6 bp genotype and habit of frequent alcohol drinking were at an increased risk of developing SC (adjusted OR = 1.76, 95% CI: 1.07 - 2.90) compared with those with +6 bp alleles and low consumption of alcohol. As compared with individuals with +6 bp alleles and who had habit of tea drinking, individuals who had TS -6 bp/-6 bp genotype and but without habit of tea drinking had an increased risk of developing SC (adjusted OR = 2.34, 95% CI: 1.43 - 3.82). (3) Individuals with TS -6 bp/-6 bp genotype and with MTHFR T alleles had an increased risk of developing SC (adjusted OR = 2.67, 95% CI: 1.07 - 6.70) compared with those with +6 bp alleles and with MTHRF C/C genotype.

CONCLUSION

Results in the present study suggested that there was a combined effect between lifestyle, MTHFR C/T or T/T genotype and TS -6 bp/-6 bp genotype in the development of SC.

摘要

目的

参照胃癌（SC）的发病情况，评估生活方式、亚甲基四氢叶酸还原酶基因（MTHFR）以及胸苷酸合成酶基因（TS）3'非翻译区（3'-UTR）多态性之间的相互作用。

方法

在中国江苏省淮安市开展了一项病例对照研究，其中包括107例胃癌病例和200例基于人群的对照。通过聚合酶链反应鉴定TS基因型。

结果

（1）病例组中TS基因型（+6bp/+6bp、+6bp/-6bp和-6bp/-6bp）的频率分别为5.6%、47.7%和46.7%，对照组中分别为9.0%、54.0%和37.0%。被鉴定为-6bp/-6bp基因型的个体患胃癌的风险略高于携带+6bp等位基因的个体（粗比值比=1.49，95%置信区间：0.90-2.47；调整后比值比=1.36，95%置信区间：1.00-1.78，P=0.047）。（2）与携带+6bp等位基因且无吸烟习惯的个体相比，携带TS -6bp/-6bp基因型且有吸烟习惯的个体患胃癌的风险显著更高（调整后比值比=2.79，95%置信区间：1.51-5.18）。与携带+6bp等位基因且饮酒量少的个体相比，携带TS -6bp/-6bp基因型且有频繁饮酒习惯的个体患胃癌的风险增加（调整后比值比=1.76，95%置信区间：1.07-2.90）。与携带+6bp等位基因且有饮茶习惯的个体相比，携带TS -6bp/-6bp基因型但无饮茶习惯的个体患胃癌的风险增加（调整后比值比=2.34，95%置信区间：1.43-3.82）。（3）与携带+6bp等位基因且为MTHFR C/C基因型的个体相比，携带TS -6bp/-6bp基因型且为MTHFR T等位基因的个体患胃癌的风险增加（调整后比值比=2.67，95%置信区间：1.07-6.70）。