酶在四氢生物蝶呤生物合成中的作用：最后一步连续反应的计算机分析

Role of enzymes in tetrahydrobiopterin biosynthesis: computer analysis of the sequential reaction of the last step.

作者信息

Katoh S, Sueoka T, Hikita H

机构信息

Deptartment of Biochemistry, Meikai University School of Dentistry, Saitama, Japan.

出版信息

J Nutr Sci Vitaminol (Tokyo). 1992;Spec No:497-500. doi: 10.3177/jnsv.38.special_497.

DOI:10.3177/jnsv.38.special_497

PMID:1297796

Abstract

Computer simulation analysis of the experimental data showed that Cl'-keto PH4 and C2'-keto PH4 were sequential intermediates of the reduction of PPH4 to BH4 by SPR and that the reaction in the last step of the BH4 biosynthesis proceeds as follows: PPH4-->Cl'-keto PH4-->C2'-keto PH4-->BH4 Cl'-keto PH4 is not detectable in the reduction of PPH4 by the purified SPR at neutral pH since isomerization (-->) [8] may occur far more rapidly than the first reduction (-->).

摘要

对实验数据的计算机模拟分析表明，Cl'-酮基-PH4和C2'-酮基-PH4是SPR将PPH4还原为BH4过程中的连续中间体，并且BH4生物合成最后一步的反应如下：PPH4→Cl'-酮基-PH4→C2'-酮基-PH4→BH4。在中性pH条件下，纯化的SPR将PPH4还原时，Cl'-酮基-PH4无法检测到，因为异构化（-->[8]）可能比首次还原（-->）快得多。