Belova L M
Parazitologiia. 1992 Sep-Oct;26(5):443-7.
The method of cultivation of Blastocystis galli from hens and Blastocystis sp. from ducks was worked out. Blastocystis grow on nutrient medium at pH 7.0 to 7.2 in a wide range of temperatures from 30 to 45 C. Optimum temperatures for cultivation are 41 to 42 C. The growth of cultures was obtained on biphase egg medium. Solid phase of the medium presents coagulated contents of the hen's egg. Liquid phase can be made of Henk's solution with the addition of 30% of fresh or lyophilizinic hen serum or horse serum. Henk's solution can be replaced by medium 199 (we observed the growth of culture on medium 199 without addition of blood serum). In all variants of medium we added antibiotics on a per--1 ml of medium basis: ampicillini--4 thousand units, streptomycini--1 thousand units. After 2 to 3 passages antibiotics can be excluded from the medium. Optimum medium is that with the addition of 30% of fresh hen serum. Passages go well at the transfer of 15-20% culture after 72 to 96 hours. The size of cultural stages varied within the limits of 2.5-56.2 x 2.5-56.2 microns and 2.5-110 x 2.5-110 microns for Blastocystis sp. and B. galli, respectively, the number of nuclei in one individual varied from 1 to 64, seldom over 100.
已摸索出从母鸡体内培养鸡囊滴虫以及从鸭体内培养某种囊滴虫的方法。囊滴虫能在pH值为7.0至7.2的营养培养基上，于30至45摄氏度的广泛温度范围内生长。培养的最适温度为41至42摄氏度。在双相鸡蛋培养基上可实现培养物的生长。培养基的固相为凝固的鸡蛋内容物。液相可用亨克氏溶液制成，并添加30%的新鲜或冻干鸡血清或马血清。亨克氏溶液可用199培养基替代（我们观察到在未添加血清的199培养基上培养物也能生长）。在所有培养基变体中，我们每1毫升培养基添加抗生素：氨苄青霉素4000单位、链霉素1000单位。传代2至3次后可从培养基中去除抗生素。最佳培养基是添加30%新鲜鸡血清的那种。在72至96小时后转移15 - 20%的培养物时传代效果良好。对于某种囊滴虫和鸡囊滴虫，培养阶段的大小分别在2.5 - 56.2×2.5 - 56.2微米和2.5 - 110×2.5 - 110微米范围内变化，单个个体中的核数从1至64不等，很少超过100。
