Anselmo R J, Viora S, Carletti S
Universidad Nacional de Luján, Argentina.
Rev Argent Microbiol. 1992 Apr-Jun;24(2):86-90.
The efficiency of xanthan production through surface processes was evaluated. The best porous material was selected first. Thereafter, a comparative study was performed using submerged agitated process vs other without agitation but containing the selected porous material. The culture medium used was white potatoes infusion, buffered with K2HPO4 and supplemented with glucose in diverse concentrations. Besides, to evaluate a different type of surface process, three vegetables were valued: Ipomaea batatus, Solanum tuberosum and Daucus carota, with an without glucose supplement. Larger xanthan production was achieved with immobilization of X. campestris vs the conventional method, when the liquid culture medium was used. The highest yield was obtained when the white potatoes infusion was supplemented with glucose 2.5%, yielding a conversion of this saccharide to xanthan up to 58%. When X. campestris was cultured on fragmented vegetables, the highest xanthan gum yield (5.6g) was obtained with Solanum tuberosum supplemented with glucose. This yield indicators that X. campestris used the glucose added as well as the constitutive polysaccharide of this vegetable.
评估了通过表面过程生产黄原胶的效率。首先选择了最佳的多孔材料。此后,进行了一项对比研究,使用深层搅拌工艺与其他不搅拌但含有所选多孔材料的工艺进行比较。所用培养基为白土豆浸出液,用磷酸氢二钾缓冲,并添加不同浓度的葡萄糖。此外,为了评估不同类型的表面过程,对三种蔬菜进行了评估:甘薯、马铃薯和胡萝卜,有添加葡萄糖和不添加葡萄糖的情况。当使用液体培养基时,与传统方法相比,通过野油菜黄单胞菌的固定化实现了更高的黄原胶产量。当白土豆浸出液添加2.5%的葡萄糖时,获得了最高产量,这种糖类转化为黄原胶的转化率高达58%。当野油菜黄单胞菌在切碎的蔬菜上培养时,添加葡萄糖的马铃薯获得了最高的黄原胶产量(5.6克)。该产量表明野油菜黄单胞菌利用了添加的葡萄糖以及这种蔬菜的组成多糖。