利用新型酶传感器测定市售药品中具有抗胆碱酯酶活性的化合物。

Determination of compounds with anticholinesterase activity in commercial drugs by a new enzyme sensor.

作者信息

Campanella L, Cocco R, Tomassetti M

机构信息

Dipartimento di Chimica, Università di Roma La Sapienza, Italy.

出版信息

J Pharm Biomed Anal. 1992 Oct-Dec;10(10-12):741-9. doi: 10.1016/0731-7085(91)80074-j.

DOI:10.1016/0731-7085(91)80074-j

PMID:1298377

Abstract

A suitable enzyme sensor for the analysis of anticholinesterase compounds of pharmaceutical interest is described. It is based on the competitive inhibiting properties of these compounds on the enzyme butyrylcholinesterase and it is constituted by a hydrogen peroxide amperometric electrode modified by a superimposed Nylon membrane containing two chemically immobilized biological mediators (butyrylcholinesterase and choline oxidase). Some applications to the analysis of several pharmaceutical forms containing different compounds showing anticholinesterase activity are also reported and evaluated.

摘要

本文描述了一种适用于分析具有药学意义的抗胆碱酯酶化合物的酶传感器。它基于这些化合物对丁酰胆碱酯酶的竞争性抑制特性，由一个过氧化氢安培电极构成，该电极由含有两种化学固定化生物介质（丁酰胆碱酯酶和胆碱氧化酶）的叠加尼龙膜修饰。还报道并评估了该传感器在分析几种含有不同抗胆碱酯酶活性化合物的药物制剂方面的一些应用。