用安培型胆碱传感器测定血清胆碱酯酶活性和丁卡因值

Determination of serum cholinesterase activity and dibucaine numbers by an amperometric choline sensor.

作者信息

Palleschi G, Lavagnini M G, Moscone D, Pilloton R, D'Ottavio D, Evangelisti M E

机构信息

Dipartimento di Scienze e Tecnologie Chimiche Universita' di Roma, Italy.

出版信息

Biosens Bioelectron. 1990;5(1):27-35. doi: 10.1016/0956-5663(90)80024-8.

DOI:10.1016/0956-5663(90)80024-8

PMID:2178637

Abstract

Serum cholinesterase activity and the dibucaine numbers have been determined by using a hydrogen peroxide electrode and the enzyme choline oxidase immobilized on a nylon net. The analysis procedure is extremely simple and very fast allowing 30 cholinesterase determinations per hour. Both cholinesterase activity and dibucaine number measurements could be performed in 5 min and by using serum samples of only 10 microliters. When used in sera the probe showed no interference from electroactive compounds present in blood, and also showed good stability and reproducibility. These features make this sensor appropriate for continuous extracorporeal circuit blood monitoring of succinylcholine during surgery.

摘要

已使用过氧化氢电极和固定在尼龙网上的胆碱氧化酶测定了血清胆碱酯酶活性和地布卡因值。分析过程极其简单且非常快速，每小时可进行30次胆碱酯酶测定。胆碱酯酶活性和地布卡因值的测量均可在5分钟内完成，且仅需10微升血清样本。该传感器用于血清时，未显示出血液中存在的电活性化合物的干扰，并且还具有良好的稳定性和重现性。这些特性使得该传感器适用于手术期间对琥珀酰胆碱进行连续体外循环血液监测。