Riikonen P, Mäkelä P H, Saarilahti H, Sukupolvi S, Taira S, Rhen M
National Public Health Institute, Molecular Biology Program, Helsinki, Finland.
Microb Pathog. 1992 Oct;13(4):281-91. doi: 10.1016/0882-4010(92)90038-p.
The virulence plasmid, characteristic of many serovars of Salmonella sp., and specifically its spv genes, promote intracellular growth of the bacteria in the liver and spleen and are essential for the virulence of these Salmonella serovars in the mouse. In an attempt to establish an in vitro model for studying its function, we evaluated its effect on the intracellular growth of the bacteria in macrophages in culture. We used a number of different macrophage-like cell lines (J774-A.1, IC-21 and PU5-1.8), as well as peritoneal or splenic macrophages from genetically Salmonella-sensitive (Itys, BALB/c) or resistant (Ityr, C3H/HeN) mice, and at different states of activation, stimulated in vivo or in vitro with lipopolysaccharide and/or recombinant gamma interferon. These were found to differ in their ability to suppress or sustain intracellular growth of several Salmonella serovars, but in all cases the growth was independent of the spv genes.
毒力质粒是许多沙门氏菌血清型的特征，特别是其spv基因，可促进细菌在肝脏和脾脏中的细胞内生长，并且对于这些沙门氏菌血清型在小鼠中的毒力至关重要。为了建立一个研究其功能的体外模型，我们评估了它对培养的巨噬细胞中细菌细胞内生长的影响。我们使用了多种不同的巨噬细胞样细胞系（J774-A.1、IC-21和PU5-1.8），以及来自对沙门氏菌遗传敏感（Itys，BALB/c）或抗性（Ityr，C3H/HeN）小鼠的腹膜或脾巨噬细胞，并处于不同的激活状态，通过体内或体外脂多糖和/或重组γ干扰素刺激。发现这些细胞系在抑制或维持几种沙门氏菌血清型细胞内生长的能力上存在差异，但在所有情况下，生长均与spv基因无关。
