Jing Y, Han R
Institute of Materia Medica, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1992 Oct;14(5):361-4.
Human promyelocytic leukemia HL-60 cells provide a useful model system for the study of cell differentiation in vitro. Here the growth of HL-60 cells as a solid clot with fibrin in subrenal capsules (SRC) of mice was studied. The cell volume of HL-60 cells increased 5 and 15-fold between 6-9 days after transplantation into normal and CYT immunosuppressed mice, respectively. Histological study revealed typical proliferation and invasion characteristics of HL-60 cells and higher tumor cell density. RA, RII, Ara-C, Harringtonine and HMBA significantly inhibited the growth of HL-60 cells in SRC of mice. This model provides a useful system for the study of the effect of drugs on cultured tumor cells or leukemia cell lines in vivo.
人早幼粒白血病HL-60细胞为体外细胞分化研究提供了一个有用的模型系统。在此，研究了HL-60细胞在小鼠肾被膜下（SRC）与纤维蛋白形成固体凝块的生长情况。移植到正常小鼠和经CYT免疫抑制的小鼠体内后，HL-60细胞的细胞体积在6 - 9天内分别增加了5倍和15倍。组织学研究揭示了HL-60细胞典型的增殖和侵袭特征以及更高的肿瘤细胞密度。维甲酸、RII、阿糖胞苷、高三尖杉酯碱和六亚甲基双乙酰胺显著抑制了HL-60细胞在小鼠SRC中的生长。该模型为研究药物对体内培养的肿瘤细胞或白血病细胞系的作用提供了一个有用的系统。
