Zeng W Z, Liu T P
Department of Pharmacology, Nanjing Medical College, China.
Zhongguo Yao Li Xue Bao. 1992 Sep;13(5):420-3.
Intracellular recordings were obtained from the cytosome of type A primary afferents in the isolated toad dorsal root ganglia (DRG) preparations. Bath application of Ca2+ 8.0 mmol.L-1 led to prolong action potential duration of repolarization 100% (APD100) by 23%, afterhyperpolarization potential duration of depolarization 50% (AHPD50) by 46%, and increase afterhyperpolarization potential amplitude (AHPA) by 40%. Verapamil 3 mumol.L-1 shortened the APD100 by 7% and the AHPD50 by 13%, and reduced the AHPA by 17%. The effects of Tet 3-100 mumol.L-1 consisted of APD and AHPD50 shortening and AHPA reduction in concentration-dependent manner. Tet 100 mumol.L-1, APD100 was shortened by 16%, AHPD50 by 18%, and AHPA was reduced by 20%. The results suggested that the effects of Tet may be related to its Ca2+ channel blockade in DRG.
在离体蟾蜍背根神经节（DRG）标本中，从 A 型初级传入纤维的胞体进行细胞内记录。浴槽中施加 8.0 mmol·L⁻¹ 的 Ca²⁺ 可使复极化动作电位持续时间 100%（APD100）延长 23%，去极化后超极化电位持续时间 50%（AHPD50）延长 46%，并使后超极化电位幅度（AHPA）增加 40%。3 μmol·L⁻¹ 的维拉帕米使 APD100 缩短 7%，AHPD50 缩短 13%，并使 AHPA 降低 17%。3 - 100 μmol·L⁻¹ 的四乙铵（Tet）的作用包括以浓度依赖方式缩短 APD 和 AHPD50 以及降低 AHPA。100 μmol·L⁻¹ 的 Tet 使 APD100 缩短 16%，AHPD50 缩短 18%，AHPA 降低 20%。结果表明，Tet 的作用可能与其对 DRG 中 Ca²⁺ 通道的阻断有关。
