Singh J, Devi S
Industrial Toxicology Research Centre, Lucknow, India.
Biotech Histochem. 1992 Sep;67(5):261-4. doi: 10.3109/10520299209110034.
The utility of fluorescence microscopy for studying development of fern spores is investigated. Changes in the fluorescence characteristics during the developmental stages of fern sporangia can be attributed to the changes in the chemical composition of the cell wall. Bright blue autofluorescence of the spores indicated the presence of sporopollenin. The sporangial walls and the spores autofluoresced yellow under blue light excitation. Fluorescence microscopy is a useful addition to light, scanning, and transmission electron microscopy because living specimens can be studied owing to their fluorescence properties.
研究了荧光显微镜在蕨类植物孢子发育研究中的效用。蕨类植物孢子囊发育阶段荧光特性的变化可归因于细胞壁化学成分的变化。孢子发出的亮蓝色自发荧光表明存在孢粉素。在蓝光激发下，孢子囊壁和孢子发出黄色自发荧光。荧光显微镜是对光学显微镜、扫描电子显微镜和透射电子显微镜的有益补充，因为可以根据活体标本的荧光特性对其进行研究。
