Kale S A, Rao S G
Cell Biology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
Nouv Rev Fr Hematol (1978). 1992;34(6):449-54.
The haematopoietic microenvironment which is provided by the stromal cells and extracellular matrix elements has been shown to influence the growth and differentiation of haematopoietic cells. However, most of the information thus far available has been derived from experiments performed using Long Term Bone Marrow Cultures and there is as yet no information regarding the haematopoietic microenvironmental status of the developing liver. Stroma were therefore established using 13-day embryonal liver cells, at a point in time when the liver shows peak haematopoietic activity. These stroma when compared to bone marrow stroma showed differences in histochemical staining. 2M NaCl extracts from 13-day embryonal liver were capable of binding pluripotent stem cells and on SDS-PAGE showed a 14 kDa band of increased intensity. The extracts could also induce erythroid colonies in a semisolid medium. However, 13-day embryonal liver stroma were unable to maintain bone marrow stem cells in culture and overall results would suggest differences between the stromal components of bone marrow and embryonal liver.
由基质细胞和细胞外基质成分提供的造血微环境已被证明会影响造血细胞的生长和分化。然而，目前所获得的大部分信息都来自于使用长期骨髓培养进行的实验，关于发育中肝脏的造血微环境状况尚无相关信息。因此，利用13天胚胎肝脏细胞建立了基质，此时肝脏呈现出造血活性高峰。与骨髓基质相比，这些基质在组织化学染色上表现出差异。13天胚胎肝脏的2M NaCl提取物能够结合多能干细胞，在SDS - PAGE上显示出一条强度增加的14 kDa条带。该提取物还能在半固体培养基中诱导红系集落形成。然而，13天胚胎肝脏基质在培养中无法维持骨髓干细胞，总体结果表明骨髓和胚胎肝脏的基质成分存在差异。
