在转基因小鼠中，与细菌氯霉素乙酰转移酶基因融合的大鼠甲状腺球蛋白启动子的甲状腺特异性和激素依赖性表达。

Thyroid-specific and hormone-dependent expression of rat thyroglobulin promoter fused with bacterial chloramphenicol acetyltransferase gene in transgenic mice.

作者信息

Skinner C A, Kelly D, Kao L R, Nayfeh S N, Popko B, Chae C B

机构信息

Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.

出版信息

Mol Cell Endocrinol. 1992 Dec;90(1):33-8. doi: 10.1016/0303-7207(92)90098-q.

DOI:10.1016/0303-7207(92)90098-q

PMID:1301397

Abstract

The minimal promoter of rat thyroglobulin (TG) gene (168 bp) was fused with bacterial chloramphenicol acetyltransferase (CAT) gene, and transgenic mice carrying the TGCAT gene were produced. The minimal promoter is sufficient for thyroid-specific and hormone-dependent expression of TGCAT in transgenic mice. Deletion of a region between -128 and -92 bp (TGII), which is not required for the expression of TGCAT in transient expression assays but whose sequence is most extensively conserved among different species, appears to decrease frequency of the expression of TGCAT in transgenic mice. However, the same deletion apparently has no significant effect on TG promoter activity in stably transformed rat FRTL-5 cells.

摘要

将大鼠甲状腺球蛋白（TG）基因的最小启动子（168bp）与细菌氯霉素乙酰转移酶（CAT）基因融合，并制备携带TGCAT基因的转基因小鼠。该最小启动子足以使TGCAT在转基因小鼠中实现甲状腺特异性和激素依赖性表达。在瞬时表达试验中，删除-128至-92bp之间的区域（TGII）对TGCAT的表达并非必需，但其序列在不同物种间最为保守，而删除该区域似乎会降低转基因小鼠中TGCAT的表达频率。然而，相同的缺失对稳定转化的大鼠FRTL-5细胞中的TG启动子活性显然没有显著影响。