Scarpini E, Doronzo R, Baron P, Moggio M, Basellini A, Scarlato G
Institute of Neurology, Dino Ferrari Center, University of Milan, Italy.
Int J Clin Pharmacol Res. 1992;12(5-6):211-5.
The phenotypic (morphologic and antigenic) properties and mitotic index of cultured Schwann cells obtained by dissecting nerves from six diabetic patients were studied. These features were compared with those of Schwann cells cultured in vitro from six normal control nerves. Preservation of the specific antigenic properties of cells, identified with rabbit antiserum as bovine protein S-100, was documented by immunofluorescence with monoclonal antibodies against laminin, fibronectin, histocompatibility antigens HLA-A, B, C and -DR, HNK-1 antigen and the human receptor for nerve growth factor. The cell proliferation index was assessed by incorporation of bromodeoxyuridine. The results of the study showed that the mitotic capacities of Schwann cells from diabetic nerves cultured in vitro remained comparable with those of normal control cells. As regards phenotypic characteristics, no modifications were detectable by immunofluorescence. These findings suggest that the phenomena of demyelination and remyelination, sometimes with onion bulb features, which can be observed in some cases of diabetic neuropathy, are not due to a primary dysfunction of the Schwann cells but are secondary to axonal degeneration.
对从6名糖尿病患者的神经分离得到的培养雪旺细胞的表型（形态学和抗原性）特性及有丝分裂指数进行了研究。将这些特征与从6条正常对照神经体外培养的雪旺细胞的特征进行了比较。用抗层粘连蛋白、纤连蛋白、组织相容性抗原HLA - A、B、C和 - DR、HNK - 1抗原以及神经生长因子人受体的单克隆抗体进行免疫荧光检测，证实了用兔抗血清鉴定为牛蛋白S - 100的细胞特异性抗原特性得以保留。通过溴脱氧尿苷掺入评估细胞增殖指数。研究结果表明，体外培养的糖尿病神经雪旺细胞的有丝分裂能力与正常对照细胞相当。就表型特征而言，免疫荧光检测未发现有改变。这些发现提示，在某些糖尿病性神经病变病例中可观察到的脱髓鞘和再髓鞘形成现象，有时伴有洋葱球样特征，并非由于雪旺细胞的原发性功能障碍，而是继发于轴突变性。
