Chang S M, Lei L F, Tian L
Academia Sinica, Shanghai Institute of Cell Biology.
Shi Yan Sheng Wu Xue Bao. 1992 Dec;25(4):317-27.
The effect of gossypol on prostate cells of rat was studied both in vitro and in vivo. For in vivo study, mature male Sprague Dawley rats were given gossypol orally, 5 mg/day, 5 times a week for one month. Then the prostates were examined histologically. By adding gossypol to the culture system directly, NbE-1 cells were used in vitro study. Several parameters, including histological structure, cell growth, DNA synthesis and mitotic cycle of cells were measured by different methods. The prostate size and weight in experimental rats decreased and significant differences between acini in controls and gossypol treated animals were noted. Acini of control prostate were full of protruding folds constituted by columnar epithelial cells, whereas, the most of the acini in treated animals were composing of cubic or square epithelial cells and epithelial folds were rarely seen. Thus, acini with normal appearance were about 14% less than that in controls. In the in vitro experiments, both cell proliferation and DNA synthesis decreased from 0 to 80% and from 0 to 90% respectively, when the concentrations of gossypol were increased from 0 to 20 micrograms/ml. It seems that 10 micrograms/ml of gossypol could cause the most significant inhibition in these two cellular functions. The cell mitotic assay showed that in treated groups, the cell number of S phase was decreased from 43 to 31%, this fact indicated that the inhibition on cell proliferation caused by gossypol might be due to preventing cells to enter S phase. Besides, inhibition effect on the proliferation of prostatic epithelial cells was dosage dependent and related to the duration of treatment.
研究了棉酚对大鼠前列腺细胞的体内外作用。在体内研究中，给成熟雄性斯普拉格-道利大鼠口服棉酚，剂量为5毫克/天，每周5次，持续1个月。然后对前列腺进行组织学检查。在体外研究中，通过直接向培养系统中添加棉酚，使用NbE-1细胞。采用不同方法测量了包括组织结构、细胞生长、DNA合成和细胞有丝分裂周期等几个参数。实验大鼠的前列腺大小和重量减小，对照组和棉酚处理组的腺泡之间存在显著差异。对照前列腺的腺泡充满由柱状上皮细胞构成的突出褶皱，而处理组动物的大多数腺泡由立方或方形上皮细胞组成，很少见到上皮褶皱。因此，外观正常的腺泡比对照组少约14%。在体外实验中，当棉酚浓度从0增加到20微克/毫升时，细胞增殖和DNA合成分别从0下降到80%和从0下降到90%。似乎10微克/毫升的棉酚对这两种细胞功能的抑制作用最为显著。细胞有丝分裂分析表明，在处理组中，S期细胞数量从43%下降到31%，这一事实表明棉酚对细胞增殖的抑制可能是由于阻止细胞进入S期。此外，对前列腺上皮细胞增殖的抑制作用呈剂量依赖性，且与治疗持续时间有关。
