Green-Johnson J M, Wade A W, Szewczuk M R
Department of Microbiology & Immunology, Queen's University, Kingston, Ontario, Canada.
Reg Immunol. 1992 May-Jun;4(3):175-85.
The proportion of memory cells expressing the Pgp-1 surface marker, and subsets of T cells expressing L3T4 (CD4+ helper cells), Lyt-2 (CD8+ suppressor/cytotoxic cells), LFA-1 (lymphocyte function-associated antigen-1), and interleukin-2 receptors (IL-2R) on the cell surface in the spleen, regional lymph nodes (PLN), mesenteric LN (MLN), bronchial or mediastinal LN (BLN), Peyer's patches (PP), thymus, and bone marrow (BM) was studied in C57BL/6J mice of varying ages. Monoclonal antibodies (Mabs) IM7.8.1, FD4, GK1.5, 3.155, and FD441.8 were used to measure Pgp-1, IL-2R, L3T4 Lyt-2, and LFA-1 expressions, respectively. Optimal dose and kinetic studies were determined. The percentages of positive cells were determined by monoclonal antibody staining and flow cytometry or immunofluorescence microscopy. Using flow cytometric analysis, we found significant age-associated increases in the percentages of Pgp-1+ cells in the MLN as compared with a slight, but not significant, increase in the spleen. There were significant age-related increases in the percentages of Lyt-2+ cells in the spleen with no change in the MLN. The percentages of cells with the other phenotypic markers, L3T4, LFA-1, and IL-2R did not change with age in the spleen or MLN. Using immunofluorescence microscopy, the percentages of Thy-1.2+, Lyt-1+, and Lyt-2+ cells in different anatomical immune tissues did not change with age, except in the BLN and PP where there were significant age-related declines of the percentages of Thy-1.2+ and Lyt-2+ cells in the BLN, and of Lyt-1+ cells in the PP. These results indicate elevated levels of Pgp-1+ memory senescent cells in the MLN and these age-related shifts or changes in T lymphocyte subsets with age could contribute to the conserved immune responsiveness of senescent mucosal T lymphocytes.
在不同年龄的C57BL/6J小鼠中，研究了脾脏、局部淋巴结（PLN）、肠系膜淋巴结（MLN）、支气管或纵隔淋巴结（BLN）、派伊尔结（PP）、胸腺和骨髓（BM）中表达Pgp-1表面标志物的记忆细胞比例，以及细胞表面表达L3T4（CD4+辅助细胞）、Lyt-2（CD8+抑制/细胞毒性细胞）、淋巴细胞功能相关抗原-1（LFA-1）和白细胞介素-2受体（IL-2R）的T细胞亚群比例。分别使用单克隆抗体（Mab）IM7.8.1、FD4、GK1.5、3.155和FD441.8来检测Pgp-1、IL-2R、L3T4、Lyt-2和LFA-1的表达。确定了最佳剂量和动力学研究。通过单克隆抗体染色和流式细胞术或免疫荧光显微镜确定阳性细胞的百分比。使用流式细胞术分析，我们发现与脾脏中轻微但不显著的增加相比，MLN中Pgp-1+细胞的百分比有显著的年龄相关性增加。脾脏中Lyt-2+细胞的百分比有显著的年龄相关性增加，而MLN中没有变化。脾脏或MLN中其他表型标志物L3T4、LFA-1和IL-2R的细胞百分比不随年龄变化。使用免疫荧光显微镜，除了BLN和PP外，不同解剖免疫组织中Thy-1.2+、Lyt-1+和Lyt-2+细胞的百分比不随年龄变化，在BLN中Thy-1.2+和Lyt-2+细胞的百分比以及在PP中Lyt-1+细胞的百分比有显著的年龄相关性下降。这些结果表明MLN中Pgp-1+记忆衰老细胞水平升高，并且这些与年龄相关的T淋巴细胞亚群的变化可能有助于衰老黏膜T淋巴细胞保守的免疫反应性。
