Scholey A B, Bullock S, Rose S P
Department of Biology, Open University, Milton Keynes, U.K.
Neurochem Int. 1992 Oct;21(3):343-50. doi: 10.1016/0197-0186(92)90185-t.
A monoclonal antibody was used to examine changes in immunoreactivity of the cytoskeletal protein, alpha-tubulin, following passive avoidance learning in day-old chicks. Postmitochondrial fractions (16,000 g supernatants) were prepared from specific forebrain loci taken at several time points after training and assayed with the anti-alpha-tubulin antibody, YL1/2. Of the regions examined, elevations in the titre of YL1/2 were found in the left intermediate hyperstriatum ventrale 1 h, 6 h and 24 h following training, in the left lobus parolfactorius 1 h following training and in the right lobus parolfactorius 6 h and 24 h following training. No training-related changes were detected in a third forebrain region, the paleostriatum augmentatum. These results regarding the cellular dynamics of memory formation in the chick confirm and expand on earlier findings from our laboratory.
使用单克隆抗体检测了雏鸡被动回避学习后细胞骨架蛋白α-微管蛋白免疫反应性的变化。在训练后的几个时间点从特定的前脑位点制备线粒体后组分（16,000g上清液），并用抗α-微管蛋白抗体YL1/2进行检测。在所检查的区域中，训练后1小时、6小时和24小时在左侧中间腹侧上纹状体、训练后1小时在左侧嗅觉叶以及训练后6小时和24小时在右侧嗅觉叶中发现YL1/2滴度升高。在第三个前脑区域古纹状体增大部未检测到与训练相关的变化。这些关于雏鸡记忆形成细胞动力学的结果证实并扩展了我们实验室早期的发现。
