体外氧合对大鼠脂肪组织脂肪生成的影响。
In vitro influence of oxygenation on lipogenesis of rat adipose tissue.
作者信息
Macfarlane C M
机构信息
Department of Chemical Pathology, Faculty of Medicine, University of Stellenbosch, Tygerberg Hospital, Republic of South Africa.
出版信息
Clin Physiol Biochem. 1992;9(2):67-73.
Pre-incubation of rat adipose tissue from ad lib fed rats in gas mixtures containing a range of oxygen concentrations from 0% oxygen to 20% oxygen showed that incorporation of isotopes of glucose into carbon dioxide and tissue were increased after pre-incubation for a period of 14-16 hours in a gas atmosphere containing 5% oxygen. This increase required the presence of 1.2 U/ml insulin in the pre-incubation medium and it was augmented by the presence of 2.8 x 10(-7) M prostaglandin F2 alpha. Incorporation into tissue was shown to be mainly into glyceride-glycerol. Similar results were obtained with isolated fat cells.
对自由采食大鼠的脂肪组织在含氧量从0%到20%的一系列气体混合物中进行预孵育，结果显示，在含5%氧气的气体环境中预孵育14 - 16小时后，葡萄糖同位素掺入二氧化碳和组织中的量增加。这种增加要求预孵育培养基中存在1.2 U/ml胰岛素，并且2.8×10⁻⁷ M前列腺素F2α的存在会增强这种增加。掺入组织中的主要是甘油酯甘油。分离的脂肪细胞也得到了类似的结果。
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