Bertelli A, Ronca F, Ronca G, Palmieri L, Zucchi R
Institute of Biological Chemistry, University of Pisa, Italy.
Drugs Exp Clin Res. 1992;18(10):431-6.
The protective effect of L-carnitine, coenzyme Q10 and their combination on haemodynamic and metabolic variables has been investigated in isolated perfused working rat hearts after 10 min of global normothermic ischaemia followed by 60 min of reperfusion. In untreated rats or in rats treated only with L-carnitine or with coenzyme Q10, this experimental condition did not induce any irreversible myocardial injury as measured by leakage of cardiac enzymes; however, it decreased some haemodynamic parameters such as cardiac output and minute work, as well as the ATP concentration and the total adenine nucleotide pool. No variations in haemodynamic and metabolic parameters were observed in the rats treated with L-carnitine plus coenzyme Q10. In the perfusate of the hearts of the rats treated with both compounds, a lower purine release (a good index of myocardial energy balance) was also obtained. Although the molecular mechanisms remain to be defined, it appears that the association of L-carnitine and coenzyme Q10 is more effective than using these compounds separately. The complementary and synergic actions of L-carnitine and coenzyme Q10 on metabolism and against peroxidation by oxygen reaction species may explain the efficacy of their association.
在离体灌注的工作大鼠心脏中，研究了左旋肉碱、辅酶Q10及其组合对血流动力学和代谢变量的保护作用。在10分钟的常温全心缺血后再灌注60分钟。在未治疗的大鼠或仅用左旋肉碱或辅酶Q10治疗的大鼠中，这种实验条件并未导致任何不可逆的心肌损伤（通过心脏酶泄漏来衡量）；然而，它降低了一些血流动力学参数，如心输出量和每分功，以及ATP浓度和总腺嘌呤核苷酸池。在用左旋肉碱加辅酶Q10治疗的大鼠中，未观察到血流动力学和代谢参数的变化。在用这两种化合物治疗的大鼠心脏灌流液中，还获得了较低的嘌呤释放（心肌能量平衡的良好指标）。尽管分子机制尚待确定，但左旋肉碱和辅酶Q10联合使用似乎比单独使用这些化合物更有效。左旋肉碱和辅酶Q10在代谢和对抗氧反应物种过氧化方面的互补和协同作用可能解释了它们联合使用的功效。
