Awaya Y, Hiyama K, Yamakido M
Department of Internal Medicine, Hiroshima University, School of Medicine, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Dec;30 Suppl:39-41.
The authors investigated methods for analysis of oncogenes and tumor suppressor genes in lung cancers and bronchial lesions from high risk patients (retired poison gas factory workers). Amplifications of C-, L-, N-myc, length of terminal repeat array (TRA), mutations of p53 gene, p53 mRNA and K-ras genes were analysed in frozen specimens of surgically resected lung cancers. Various lesions including dysplasia, squamous metaplasia, goblet cell metaplasia, and basal cell hyperplasia were detected in the bronchial epithelium of biopsied specimens from retired poison gas factory workers. Analysis of p53 gene and k-ras gene mutations was performed on these formalin fixed, paraffin embedded samples, but no evidence of mutation has been found to date.
作者研究了高危患者（退休毒气厂工人）肺癌和支气管病变中癌基因和肿瘤抑制基因的分析方法。在手术切除肺癌的冰冻标本中分析了C-、L-、N-myc的扩增、末端重复序列阵列（TRA）的长度、p53基因、p53 mRNA和K-ras基因的突变情况。在退休毒气厂工人活检标本的支气管上皮中检测到了包括发育异常、鳞状化生、杯状细胞化生和基底细胞增生在内的各种病变。对这些经福尔马林固定、石蜡包埋的样本进行了p53基因和k-ras基因突变分析，但迄今为止未发现突变证据。
