通过化疗促进产生LAK吸引剂，增强转移的淋巴因子激活的杀伤细胞（LAK细胞）在小鼠肿瘤部位的聚集。

Augmented accumulation of transferred lymphokine-activated killer (LAK) cells at murine tumor sites through production of LAK-attractant facilitated by chemotherapy.

作者信息

Hosokawa M, Wakizaka Y, Kuramitsu Y, Micallef M, Togashi Y, Kobayashi H

机构信息

Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

Tohoku J Exp Med. 1992 Oct;168(2):413-6. doi: 10.1620/tjem.168.413.

DOI:10.1620/tjem.168.413

PMID:1306328

Abstract

We observed that effects of adoptive immunotherapy with lymphokine-activated killer (LAK) cells on BMT-11, a fibrosarcoma in C57BL/6 mice were improved by combination with cyclophosphamide (CY)-chemotherapy corresponding to enhanced accumulation at tumor sites of LAK cells. On the other hand, cytotoxic T lymphocytes (CTLs) which were able to accumulate at tumor sites more densely than LAK cells produced significant therapeutic effects by themselves. We have also found observed that LAK-attractant activity was detected in conditioned medium (CM) of CY-treated tumor tissue but not in the CM of untreated tumor tissue. These findings reveal that CY-chemotherapy facilitates LAK-attractant-production and enhances the accumulation in tumor tissue of LAK cells and that therapeutic effects of adoptive transfer of LAK cells are augmented by cancer chemotherapy through the enhanced accumulation of LAK cells.

摘要

我们观察到，在C57BL/6小鼠的纤维肉瘤BMT-11中，与环磷酰胺（CY）化疗联合使用时，淋巴因子激活的杀伤（LAK）细胞过继性免疫疗法的效果得到改善，这与LAK细胞在肿瘤部位的积累增加相对应。另一方面，能够比LAK细胞更密集地聚集在肿瘤部位的细胞毒性T淋巴细胞（CTL）自身产生了显著的治疗效果。我们还发现，在CY处理的肿瘤组织的条件培养基（CM）中检测到LAK吸引活性，而在未处理的肿瘤组织的CM中未检测到。这些发现表明，CY化疗促进LAK吸引剂的产生，并增强LAK细胞在肿瘤组织中的积累，并且通过增强LAK细胞的积累，癌症化疗增强了LAK细胞过继转移的治疗效果。