Warnatz H, Lackner D, Eder E, Scheiffarth F, Gruhl M, Bischoff O
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1976 Feb 25;85(2):181-94. doi: 10.1007/BF00304951.
The in vitro cytotoxicity of lymphocytes from patients suffering from breast carcinoma against autochthonous, allogeneic and established breast carcinoma cells was evaluated. Lymphocytotoxicity to breast carcinoma cells was observed in all stages of the disease. Control lymphocytes from healthy donors or patients suffering from other carcinoma are not cytotoxic for the breast carcinoma cell lines. A follow-up study of the cell mediated immune reactions before and after surgical removal of the breast carcinoma showed that the cytotoxic lymphocyte population which is demonstrated in the presence of the tumor disappears quickly after excision of the carcinoma. The non-reactivity of lymphocytes is not due to a general immune defect. Serum of the tumor bearers did not block the lymphocytotoxicity in early stage breast carcinoma; in metastatic disease inhibition occurred in more than half of the cases. Preincubation of lymphocytes with antigen preparations of allogeneic breast carcinoma cells did not inhibit the cytotoxicity to breast carcinoma cells whereas autologous serum preincubated with the antigen preparation diminished the lymphocyte reactivity to the target cells in some cases.
对乳腺癌患者淋巴细胞针对自身、异体及已建立的乳腺癌细胞的体外细胞毒性进行了评估。在疾病的各个阶段均观察到淋巴细胞对乳腺癌细胞的细胞毒性。来自健康供体或患有其他癌症的患者的对照淋巴细胞对乳腺癌细胞系无细胞毒性。对乳腺癌手术切除前后细胞介导免疫反应的随访研究表明，在肿瘤存在时表现出的细胞毒性淋巴细胞群体在癌切除后迅速消失。淋巴细胞的无反应性并非由于普遍的免疫缺陷。肿瘤携带者的血清在早期乳腺癌中并未阻断淋巴细胞毒性；在转移性疾病中，超过半数的病例出现抑制现象。淋巴细胞与异体乳腺癌细胞抗原制剂预孵育并未抑制对乳腺癌细胞的细胞毒性，而自体血清与抗原制剂预孵育在某些情况下会降低淋巴细胞对靶细胞的反应性。
