Anantaraman S, Ravindranath M H
Z Parasitenkd. 1976 Feb 6;48(3-4):227-38. doi: 10.1007/BF00380396.
The histochemical and phase contrast observations on the embryonic envelopes enclosing the acanthor of Acanthosentis sp. reveal the presence of three layers. All the three layers were non-refractile during early stages of their formation, and become refringent subsequently. The outer layer loses its refractile property together with affinity for acid and basic dyes when the eggs are fully developed. This layer is neither tanned nor chitinous. The second layer consists of glyco-lipo-proteinous matrix. The polysaccharide moiety is identified to be strongly sulfated acid mucopolysaccharide. The lipid component has been characterized to be KOH resistant and esters of unsaturated fatty acid. The protein is rich in aromatic amino acids. The third and the innermost layer contains chitin and acid mucopoly-saccharides. The protein is rich in sulfur-containing amino acids. Sulphydryl groups were present during early stages of its formation, but forms disulphide cross-links subsequently. Stages in the development of acanthors were characterized with reference to the formation of envelopes. The permeability experiments carried out on fresh eggs using 0.1% aqueous solutions of bromophenol blue, methylene blue, toluidine blue, basic fuchsin and neutral red indicate that the inward diffusion of these substances is prevented subsequent to formation of disulphide groups in the innermost layer. The results further indicate that the embryo derives nutritive requirements from the fluid of pseudocoel of the female worm through th envelopes covering it.
对棘头虫属棘头蚴周围胚胎包膜的组织化学和相差显微镜观察显示存在三层结构。在其形成的早期阶段，所有这三层均无折光性，随后变得有折光性。当卵完全发育时，外层失去其折光性以及对酸性和碱性染料的亲和力。这一层既非鞣制而成也不是几丁质的。第二层由糖脂蛋白基质组成。多糖部分被鉴定为强硫酸化酸性粘多糖。脂质成分的特征是耐氢氧化钾且为不饱和脂肪酸的酯。蛋白质富含芳香族氨基酸。第三层也是最内层含有几丁质和酸性粘多糖。蛋白质富含含硫氨基酸。在其形成的早期阶段存在巯基，但随后形成二硫键交联。棘头蚴发育阶段根据包膜的形成来表征。使用溴酚蓝、亚甲蓝、甲苯胺蓝、碱性品红和中性红的0.1%水溶液对新鲜卵进行的渗透性实验表明，在最内层形成二硫键基团后，这些物质的向内扩散受到阻止。结果进一步表明，胚胎通过覆盖它的包膜从雌虫的假体腔液中获取营养需求。
