Ling J Q
Dental Hospital, Hubei Medical College, Wuhan.
Zhonghua Kou Qiang Yi Xue Za Zhi. 1992 Nov;27(6):326-9, 384.
In the present study, DNA G+C mol% of 9 strains of bacteria (including 7 international standard strain, Ingbritt, and 2 other strains extracted in our Lab) were detected with application of High Performance Liquid Chromatograph. The results showed that DNA G+C content of S. mutans was 35-40%, S. sobrinus was 45-46%, S. cricetus was 38%, and S. rattus was 41%. The difference between S sobrinus and S. mutans was higher than 5%, which suggested that S. sobrinus should be identified as an independent classification unit of bacterial species from S. mutans.
在本研究中，应用高效液相色谱仪检测了9株细菌（包括7株国际标准菌株英布里特以及我们实验室提取的另外2株菌株）的DNA G+C摩尔百分比。结果显示，变形链球菌的DNA G+C含量为35%-40%，远缘链球菌为45%-46%，仓鼠链球菌为38%，鼠链球菌为41%。远缘链球菌与变形链球菌之间的差异高于5%，这表明远缘链球菌应被鉴定为与变形链球菌不同的独立细菌分类单元。
