Schöbitz B, Holsboer F, Kikkert R, Sutanto W, De Kloet E R
Max-Planck-Institute for Psychiatry, Clinical Institute, Munich, FRG.
Endocr Regul. 1992 Sep;26(3):103-9.
The distribution of IL-6 mRNA in the rat brain was studied by in situ hybridization using 35S-labelled oligonucleotides. The mRNA encoding IL-6 was found in hippocampus, hypothalamus, cerebellum as well as in other brain areas. Microscopic analyses revealed both neuronal and glial cell localization of the mRNA. Subsequently, Northern blot analyses were performed with RNA isolated from spleen, adrenals, pituitary gland, hypothalamus, hippocampus and cerebellum of rats injected intraperitoneally with a non toxic dose of LPS. A rapid induction of the IL-6 mRNA was observed in the peripheral organs, whereas no change in IL-6 transcripts could be measured in the brain. It is concluded that the local synthesis and release of IL-6 in pituitary and adrenal gland might be involved in the activation of the HPA axis following an endotoxin challenge.
使用35S标记的寡核苷酸通过原位杂交研究了白细胞介素-6（IL-6）mRNA在大鼠脑中的分布。在海马体、下丘脑、小脑以及其他脑区发现了编码IL-6的mRNA。显微镜分析揭示了mRNA在神经元和神经胶质细胞中的定位。随后，对腹腔注射无毒剂量脂多糖（LPS）的大鼠的脾脏、肾上腺、垂体、下丘脑、海马体和小脑中分离出的RNA进行了Northern印迹分析。在外周器官中观察到IL-6 mRNA的快速诱导，而脑中的IL-6转录本没有变化。得出的结论是，垂体和肾上腺中IL-6的局部合成和释放可能参与内毒素刺激后下丘脑-垂体-肾上腺（HPA）轴的激活。
