明胶颗粒凝集试验（MLPA）在麻风病高度流行地区用于麻风病快速血清学诊断的效用。

Utility of gelatin particle agglutination test (MLPA) for rapid serodiagnosis of leprosy in a hyperendemic area.

作者信息

Reddy B S, Badrinath S, Shantaraman R, Harish B N, Sheriff M O, Rao R S, Garg B R

机构信息

STD Department, Jawaharlal Institute of Postgraduate Medical Education & Research Pondicherry.

出版信息

Indian J Lepr. 1992 Oct-Dec;64(4):469-73.

PMID:1308521

Abstract

The anti-PGL M. leprae specific antibodies were estimated by MLPA test in 79 patients of leprosy, 8 contacts of lepromatous cases and 10 healthy controls in a hyperendemic area. The results indicated an over all seropositivity of 50.6% in leprosy patients. Three of the eight contacts and five of the controls also gave positive results. Higher seropositivity rates were noted in multibacillary patients (73% in lepromatous, 53.6% in borderline, 40% each in tuberculoid and indeterminate and 10% in pure neuritic types). The practical application of MLPA test in its present form as a serodiagnostic procedure for screening subclinical or clinical infections in leprosy patients appear to be of limited value in hyperendemic areas. Further studies involving large series of subjects are necessary for reaching definite conclusions.

摘要

在一个麻风病高度流行地区，通过多重连接探针扩增（MLPA）试验对79例麻风病患者、8例瘤型麻风病例的接触者以及10名健康对照者进行了抗麻风分支杆菌特异性抗体检测。结果显示，麻风病患者的总体血清阳性率为50.6%。8名接触者中有3人、10名对照者中有5人检测结果也呈阳性。多菌型患者的血清阳性率更高（瘤型为73%，界线类为53.6%，结核样型和未定类各为40%，纯神经炎型为10%）。MLPA试验目前作为一种血清学诊断方法用于筛查麻风病患者的亚临床或临床感染，在高度流行地区的实际应用价值似乎有限。需要开展涉及大量研究对象的进一步研究才能得出明确结论。