Horak A, Zalik S
Biochim Biophys Acta. 1976 Apr 9;430(1):135-44. doi: 10.1016/0005-2728(76)90229-2.
Spinach chloroplasts were able to photophosphorylate the ADP analog alpha,beta-methylene adenosine 5'-diphosphate (AOPCP). Phosphorylation of AOPCP was catalyzed by chloroplasts that were washed or dialyzed to remove free endogenous nucleotides. In the presence of glucose, hexokinase, AOPCP and 32Pi, the 32P label was incorporated into alpha,beta-methylene adenosine 5'-triphosphate (AOPCPOP). In contrast to photophosphorylation of AOPCP, the ATP analog AOPCPOP was a poor substrate for the ATP-Pi exchange reaction and its hydrolysis was neither stimulated by light and dithiothreitol nor inhibited by Dio-9. Photophosphorylation of AOPCP was inhibited by the alpha,beta- and beta,gamma-substituted methylene analogs of ATP, while phosphorylation of ADP was unaffected by them. The ATP-Pi exchange was also unaffected by both ATP analogs, while the weak AOPCPOP-Pi exchange was inhibited by the beta,gamma-methylene analog of ATP. Direct interaction of methylene analogs with the chloroplast coupling factor ATPase was indicated by the enzymatic hydrolysis of AOPCPOP on polyacrylamide gels.
菠菜叶绿体能够使ADP类似物α,β-亚甲基腺苷5'-二磷酸（AOPCP）发生光合磷酸化。AOPCP的磷酸化由经过洗涤或透析以去除游离内源性核苷酸的叶绿体催化。在葡萄糖、己糖激酶、AOPCP和32Pi存在的情况下，32P标记被掺入α,β-亚甲基腺苷5'-三磷酸（AOPCPOP）中。与AOPCP的光合磷酸化不同，ATP类似物AOPCPOP是ATP - Pi交换反应的不良底物，其水解既不受光和二硫苏糖醇的刺激，也不受Dio - 9的抑制。AOPCP的光合磷酸化受到ATP的α,β-和β,γ-取代亚甲基类似物的抑制，而ADP的磷酸化则不受它们的影响。两种ATP类似物对ATP - Pi交换也没有影响，而较弱的AOPCPOP - Pi交换则受到ATP的β,γ-亚甲基类似物的抑制。聚丙烯酰胺凝胶上AOPCPOP的酶促水解表明亚甲基类似物与叶绿体偶联因子ATP酶存在直接相互作用。
