Mito T, Ishiguro S, Tamai M
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
Invest Ophthalmol Vis Sci. 1992 Jan;33(1):172-7.
We studied ouabain-sensitive Na-K adenosine triphosphatase (ATPase) activities in the iris-ciliary body of rabbit eyes after lensectomy-vitrectomy. Changes in enzyme activities were quantitatively investigated in the plasma membrane of iris-ciliary body at 0 or 7 hours and at days 1, 3, 7, and 14. The specific activity of Na-K ATPase rose to significantly higher levels than the control value at 7 hours following surgery, but returned to the baseline value after 7 days. In addition, we evaluated enzyme activities after lensectomy-vitrectomy during which SF6 or silicone oil was injected. The specific activity of Na-K ATPase following the injection of SF6 or silicone oil was significantly higher than the control value at 7 hours and did not return to the normal value even after 14 days. Consensual reaction, demonstrated by increased Na-K ATPase activity, also was found in the contralateral unoperated eyes of SF6- and silicone oil-injected rabbits. The increased Na-K ATPase activity in the iris-ciliary body after experimental surgery may play an important role in restoring swollen tissues.
我们研究了晶状体切除-玻璃体切除术后兔眼虹膜睫状体中哇巴因敏感的钠钾腺苷三磷酸酶（ATP酶）活性。在术后0或7小时以及第1、3、7和14天，对虹膜睫状体的质膜中酶活性的变化进行了定量研究。钠钾ATP酶的比活性在手术后7小时升至显著高于对照值的水平，但在7天后恢复到基线值。此外，我们评估了在晶状体切除-玻璃体切除术中注入六氟化硫（SF6）或硅油后的酶活性。注入SF6或硅油后，钠钾ATP酶的比活性在7小时时显著高于对照值，甚至在14天后也未恢复到正常值。在注入SF6和硅油的兔子的对侧未手术眼中也发现了由钠钾ATP酶活性增加所证明的交感反应。实验性手术后虹膜睫状体中钠钾ATP酶活性的增加可能在肿胀组织的恢复中起重要作用。
