Sawitzke J A, Stahl F W
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229.
Genetics. 1992 Jan;130(1):7-16. doi: 10.1093/genetics/130.1.7.
The RecF pathway catalyzes generalized recombination in Escherichia coli that is mutant for recBC, sbcB and sbcC. This pathway operating on conjugational recombination requires the recA, recF, recJ, recN, recO, recQ, recR, ruvA, ruvB and ruvC genes. In contrast, lambda mutant for its own recombination genes, int, red alpha and red beta, requires only the recA and recJ genes to recombine efficiently in recBC sbcB sbcC cells. Deletion of an open reading frame in the ninR region of lambda results in an additional requirement for recO, recR and recF in order to recombine in recBC sbcB sbcC mutant cells. This function, designated orf for recO-, recR- and recF-like function, is largely RecF pathway specific.
RecF途径催化recBC、sbcB和sbcC基因发生突变的大肠杆菌中的普遍重组。这种作用于接合重组的途径需要recA、recF、recJ、recN、recO、recQ、recR、ruvA、ruvB和ruvC基因。相比之下,其自身重组基因int、redα和redβ发生突变的λ噬菌体,在recBC sbcB sbcC细胞中高效重组仅需要recA和recJ基因。λ噬菌体ninR区域中一个开放阅读框的缺失导致在recBC sbcB sbcC突变细胞中重组时对recO、recR和recF有额外需求。这种功能,因其类似于recO、recR和recF的功能而被命名为orf,在很大程度上是RecF途径特异性的。