Zgaga-Griesz A, Woscholski R, Straub P, Hug H, Marmé D
University of Freiburg, Institute of Molecular Cell Biology, FRG.
Biochem Biophys Res Commun. 1992 Jan 31;182(2):947-52. doi: 10.1016/0006-291x(92)91823-9.
Binding studies using the calcium channel blockers omega-conotoxin and dihydropyridine revealed a rather equal amount of binding sites in brain from adult chicken. The omega-conotoxin binding sites could be solubilized using digitonin, without substantial loss, whereas a great decrease in dihydropyridine binding sites was observed, indicating that both types of binding sites have different sensitivity to solubilization. In contrast to ion exchange chromatography where both binding sites comigrated, glycoprotein affinity chromatography led to a different partition of the binding sites in the flow through and eluate fractions. Our results indicate that both types of calcium channel blockers bind to different targets in adult chicken.
使用钙通道阻滞剂ω-芋螺毒素和二氢吡啶进行的结合研究表明，成年鸡脑中的结合位点数量相当。ω-芋螺毒素结合位点可用洋地黄皂苷溶解，且损失不大，而二氢吡啶结合位点则显著减少，这表明两种类型的结合位点对溶解的敏感性不同。与离子交换色谱中两种结合位点共迁移不同，糖蛋白亲和色谱导致结合位点在流出液和洗脱液组分中的分配不同。我们的结果表明，两种类型的钙通道阻滞剂在成年鸡中结合不同的靶点。
