Loret S M, Devos P E
Département de Biologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Cell Tissue Res. 1992 Jan;267(1):105-11. doi: 10.1007/BF00318696.
R*-cells of the digestive gland of Carcinus maenas have been investigated functionally and morphologically. A comparison of the capacity of separated cell suspensions to synthesize glycogen gave support to the hypothesis that R and R* cells belong to the same cell line. The unexpected observation of R* cells in gastric juice suggests that their release could represent a mode of redistribution of carbohydrate stores when the feeding activity of the crab is lower. Under electron microscopy, the calcospherites of R* cells appeared to be surrounded by multiple membranous layers, and displayed tubular and vesicular structures in their core. High glucose-6-phosphatase (G6Pase) activity in the subcellular fraction that is enriched in calcospherites suggests that these membranes are derived from the endoplasmic reticulum, via a process in which the enzyme plays a key role. We propose that this is the way by which the R cell differentiates into R* cell.
对海蟹的消化腺R细胞进行了功能和形态学研究。对分离的细胞悬液合成糖原能力的比较支持了R细胞和R细胞属于同一细胞系的假说。在胃液中意外观察到R细胞，这表明当蟹的摄食活动较低时，它们的释放可能代表碳水化合物储存重新分配的一种方式。在电子显微镜下，R细胞的钙球似乎被多层膜包围，并且在其核心显示出管状和囊泡状结构。富含钙球的亚细胞组分中高葡萄糖-6-磷酸酶（G6Pase）活性表明，这些膜是通过该酶起关键作用的过程从内质网衍生而来。我们认为这就是R细胞分化为R*细胞的方式。
