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异柠檬酸脱氢酶激酶/磷酸酶：选择性抑制磷酸酶活性的突变的鉴定。

Isocitrate dehydrogenase kinase/phosphatase: identification of mutations which selectively inhibit phosphatase activity.

作者信息

Ikeda T P, Houtz E, LaPorte D C

机构信息

Department of Biochemistry, University of Minnesota, Minneapolis 55455-0347.

出版信息

J Bacteriol. 1992 Feb;174(4):1414-6. doi: 10.1128/jb.174.4.1414-1416.1992.

DOI:10.1128/jb.174.4.1414-1416.1992
PMID:1310504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206440/
Abstract

Mutations in aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase, which selectively inhibit phosphatase activity have been isolated. These mutations yield amino acid substitutions within a 113-residue region of this 578-residue protein. These mutations may define a regulatory domain of this protein.

摘要

编码异柠檬酸脱氢酶激酶/磷酸酶的基因aceK发生了突变，这些突变可选择性抑制磷酸酶活性。这些突变导致该578个氨基酸残基的蛋白质中一个113个氨基酸残基区域内发生了氨基酸替换。这些突变可能界定了该蛋白质的一个调节结构域。

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1
Isocitrate dehydrogenase kinase/phosphatase: identification of mutations which selectively inhibit phosphatase activity.异柠檬酸脱氢酶激酶/磷酸酶：选择性抑制磷酸酶活性的突变的鉴定。
J Bacteriol. 1992 Feb;174(4):1414-6. doi: 10.1128/jb.174.4.1414-1416.1992.
2
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J Bacteriol. 1988 Jan;170(1):89-97. doi: 10.1128/jb.170.1.89-97.1988.
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The "catalytic" triad of isocitrate dehydrogenase kinase/phosphatase from E. coli and its relationship with that found in eukaryotic protein kinases.来自大肠杆菌的异柠檬酸脱氢酶激酶/磷酸酶的“催化”三联体及其与真核蛋白激酶中发现的三联体的关系。
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Mutation of the predicted ATP binding site inactivates both activities of isocitrate dehydrogenase kinase/phosphatase.
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Isocitrate dehydrogenase kinase/phosphatase: aceK alleles that express kinase but not phosphatase activity.异柠檬酸脱氢酶激酶/磷酸酶：表达激酶活性但不表达磷酸酶活性的aceK等位基因。
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A single gene codes for the kinase and phosphatase which regulate isocitrate dehydrogenase.一个单一基因编码调节异柠檬酸脱氢酶的激酶和磷酸酶。
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Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.编码异柠檬酸脱氢酶激酶/磷酸酶的基因aceK的核苷酸序列。
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引用本文的文献

1
Structural and mechanistic insights into the bifunctional enzyme isocitrate dehydrogenase kinase/phosphatase AceK.异构柠檬酸脱氢酶激酶/磷酸酶 AceK 的结构和机制见解。
Philos Trans R Soc Lond B Biol Sci. 2012 Sep 19;367(1602):2656-68. doi: 10.1098/rstb.2011.0426.

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Synthesis of C4-dicarboxylic acids from acetate by a glyoxylate bypass of the tricarboxylic acid cycle.通过三羧酸循环的乙醛酸旁路由乙酸盐合成C4 - 二羧酸。
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