Pagano M, Draetta G, Jansen-Dürr P
European Molecular Biology Laboratory, Heidelberg, Germany.
Science. 1992 Feb 28;255(5048):1144-7. doi: 10.1126/science.1312258.
The transcription factor E2F controls the expression of several proliferation-related genes and is a target of the adenovirus E1A oncogene. In human cells, both cyclin A and the cdk2 protein kinase were found in complexes with E2F. Although the total amounts of cdk2 were constant in the cell cycle, binding to E2F was detected only when cells entered S phase, a time when the cdk2 kinase is activated. These data suggest that the interaction between cdk2 and E2F requires an active kinase that has cyclin A as a targeting component.
转录因子E2F控制着多个与增殖相关基因的表达，并且是腺病毒E1A癌基因的作用靶点。在人类细胞中，细胞周期蛋白A和cdk2蛋白激酶均被发现与E2F形成复合物。尽管cdk2的总量在细胞周期中保持恒定，但只有当细胞进入S期（此时cdk2激酶被激活）时，才能检测到其与E2F的结合。这些数据表明，cdk2与E2F之间的相互作用需要一种以细胞周期蛋白A作为靶向成分的活性激酶。
