自发性高血压大鼠下丘脑和脑干中的心钠素信使核糖核酸水平升高。
Increased level of atrial natriuretic peptide messenger RNA in the hypothalamus and brainstem of spontaneously hypertensive rats.
作者信息
Komatsu K, Tanaka I, Funai T, Ichiyama A, Yoshimi T
机构信息
Second Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
出版信息
J Hypertens. 1992 Jan;10(1):17-23. doi: 10.1097/00004872-199201000-00004.
OBJECTIVE
The aim of this study was to investigate atrial natriuretic peptide (ANP) gene expression in the central nervous system (CNS) during hypertension.
METHODS
We measured and compared immunoreactive atrial natriuretic peptide (irANP) and ANP messenger RNA (mRNA) in the hypothalamus and brainstem of 17-week-old spontaneously hypertensive rats (SHR) with those of age-matched Wistar-Kyoto (WKY) rats using ribonuclease (RNase) protection assay for ANP mRNA and a specific radioimmunoassay for irANP.
RESULTS
RNase protection assay revealed that the concentrations of ANP mRNA in the hypothalamus and brainstem of SHR were higher than those of WKY rats. IrANP concentrations in the hypothalamus and brainstem of SHR were determined by a specific radioimmunoassay and found to be higher than those of WKY rats. Elevated mRNA levels in the hypothalamus and brainstem of SHR indicated that increased level of irANP in the CNS resulted from increased synthesis of ANP.
CONCLUSION
We propose that increased synthesis of brain ANP in SHR may reflect a compensatory mechanism induced by hypertension.
目的
本研究旨在调查高血压期间中枢神经系统（CNS）中的心钠素（ANP）基因表达。
方法
我们使用针对ANP mRNA的核糖核酸酶（RNase）保护测定法和针对免疫反应性心钠素（irANP）的特异性放射免疫测定法，测量并比较了17周龄自发性高血压大鼠（SHR）与年龄匹配的Wistar-Kyoto（WKY）大鼠下丘脑和脑干中的irANP和ANP信使核糖核酸（mRNA）。
结果
RNase保护测定显示，SHR下丘脑和脑干中ANP mRNA的浓度高于WKY大鼠。通过特异性放射免疫测定法测定SHR下丘脑和脑干中的irANP浓度，发现其高于WKY大鼠。SHR下丘脑和脑干中mRNA水平升高表明，CNS中irANP水平升高是由ANP合成增加所致。
结论
我们提出，SHR中脑ANP合成增加可能反映了高血压诱导的一种代偿机制。
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