大鼠肺脏含有盐皮质激素受体。

Rat lung possesses the mineralocorticoid receptor.

作者信息

Agarwal M K, Mirshahi M

机构信息

Centre Universitaire des Cordeliers, Paris, France.

出版信息

Biochem Biophys Res Commun. 1992 Mar 16;183(2):405-10. doi: 10.1016/0006-291x(92)90495-7.

DOI:10.1016/0006-291x(92)90495-7

PMID:1312828

Abstract

Lung cytosol from male, adrenalectomized rats was screened for the mineralocorticoid receptor (MCR) by a polyclonal antiserum raised in the rabbit against rat renal antigen. Western blot analysis revealed a single 98 kDa band, like the MCR purified biochemically. The MCR could also be photolabelled for the first time by 3H-R 5020 in this very 98 kDa region that was displaced by RU 26752 specific to MCR. Immune IgG was able to precipitate the MCR-3H-RU 26752 complex, and to displace the same to high molecular weight regions during gel permeation chromatography on Sephacryl columns. Thus, MCR mediated actions need to be redefined. Furthermore, the technique of photochemical labelling forms a novel tool to assess MCR specificity, and to dissect its structure and function.

摘要

用针对大鼠肾脏抗原在兔体内产生的多克隆抗血清，对雄性去肾上腺大鼠的肺胞质溶胶进行盐皮质激素受体（MCR）筛选。蛋白质印迹分析显示有一条单一的98 kDa条带，与经生化方法纯化的MCR相似。在这个98 kDa区域，MCR首次也能用³H-R 5020进行光标记，该区域能被MCR特异性的RU 26752取代。免疫IgG能够沉淀MCR-³H-RU 26752复合物，并在Sephacryl柱上进行凝胶渗透色谱分析时将其转移到高分子量区域。因此，需要重新定义MCR介导的作用。此外，光化学标记技术形成了一种评估MCR特异性以及剖析其结构和功能的新工具。